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Identification of Serpula lacrymans and other decay fungi in construction timber by sequencing of ribosomal DNA – A practical approach
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N. Högberg
Veröffentlicht/Copyright:
1. Juni 2005
Abstract
We have approached species identification of wood decay fungi in construction wood by means of sequencing ribosomal DNA. Sequencing of the Internal Transcribed Spacer (ITS), which includes the 5.8S rDNA, is straightforward and provides a basis for species identification. Identification was either by BLAST search on sequences in GenBank or phylogenetic analysis. A number of important wood decay fungi such as Serpula lacrymans, S. himantioides, Antrodia serialis, A. sinuosa, Gloeophyllum sepiarium, Fomitopsis pinicola, Resinicium bicolor and Junghuhnia collabens have been successfully identified from fruitbodies and directly from wood samples. Sequence variants were found within the species sampled including S. himantioides, the close relative of S. lacrymans. But, among 27 samples from fruitbodies and mycelium of S. lacrymans, unexpectedly, no sequence variation was detected.
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Published Online: 2005-06-01
Published in Print: 2004-03-15
Copyright © 2004 by Walter de Gruyter GmbH & Co. KG
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Artikel in diesem Heft
- Variation in content and composition of lignin in young wood of Norway spruce
- Polyoxometalate oxidation of non-phenolic lignin subunits in water: Effect of substrate structure on reaction kinetics
- Organosolv pulping of olive tree trimmings by use of ethylene glycol/soda/water mixtures
- Cooperativity analysis of the in situ lignin glass transition
- Fitting parameters for softening of wet wood under quasi static loading
- Measurement of moisture content profiles during drying of Scots pine using magnetic resonance imaging
- Influence of temperature on the modulus of elasticity (MOE) of Pinus sylvestris L.
- A contact free measurement method to determine internal stress states in glulam
- Internal stresses in the cross-grain direction in glulam induced by climate variations
- Variation in microfibril angle in Eucalyptus clones
- Durability of untreated Norway spruce (Picea abies) exposed outdoors above ground for nine years
- Microbial colonizers of freshly sawn yellow-poplar (Liriodendron tulipifera L.) lumber in two seasons: Part 1. Fungi
- Microbial colonizers of freshly sawn yellow-poplar (Liriodendron tulipifera L.) lumber in two seasons: Part 2. Bacteria
- Antifungal activity of constituents from the heartwood of Gmelina arborea: Part 1. Sensitive antifungal assay against Basidiomycetes
- Identification of volatile organic compounds (VOCs) from bacteria and yeast causing growth inhibition of sapstain fungi
- Identification of Serpula lacrymans and other decay fungi in construction timber by sequencing of ribosomal DNA – A practical approach
- Wood/plastic ratio: Effect on performance of borate biocides against a brown rot fungus
- Book Review
- Personalia
