Solid State NMR Analysis of β-13C-Enriched Lignocellulosic Material During Light-Induced Yellowing
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Jim Parkås
Summary
Photoyellowing of lignocellulosic materials has been studied with a new technique based on solid state 13C-NMR analysis of 13C-enriched DHP in cell wall tissue. The selectively 13C-enriched cell wall-dehydrogenation polymer (CW-DHP) was prepared directly on differentiating xylem from spruce (Picea abies) at pH 6.0 by administering β-13C-enriched coniferin in an enzymatic system consisting of glucose oxidase, β-glucosidase, and the naturally occurring water-insoluble enzymes remaining in the cell wall. The bonding pattern of the formed CW-DHP was found to be: 42% β-β, β-5, and β-1 substructures; 36% β-O-4 derived substructures; and 22% coniferyl alcohol and coniferaldehyde end-groups. The 13C-NMR analysis of unirradiated and irradiated tissue revealed a decrease in the relative amount of coniferaldehyde and/or coniferyl alcohol end-groups during irradiation. Prolonged irradiation also introduced new signals centered at 37, 70, and 102 ppm. The results indicate that the present technique, with the formation of DHP in a naturally lignifying carbohydrate environment, has the potential of being a valuable tool for the study of structural changes of lignin during light-induced yellowing.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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- N-Hydroxy Compounds as New Internal Standards for the 31P-NMR Determination of Lignin Hydroxy Functional Groups
- 19F NMR Spectroscopy for the Quantitative Analysis of Carbonyl Groups in Lignins
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- Experimental Determination of Stiffness Variation Across Growth Rings in Picea abies
- Determination of the Material Property Variations Across the Growth Ring of Softwood for Use in a Heterogeneous Drying Model Part 1. Capillary Pressure,Tracheid Model and Absolute Permeability
- Surface Area Determinations in Woodpulps by Humidity Adsorption
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- Literature Reports
Articles in the same Issue
- Characterisation of Fungal Degraded Birch Wood by FTIR and Py-GC
- The Effects of Specific Volatile Organic Compounds Produced by Trichoderma Spp. on the Growth of Wood Decay Basidiomycetes
- Biological Control of Blue Stain in Pulpwood: Mechanisms of Control used by Phlebiopsis gigantea
- Cell-Wall-Associated Peroxidases from the Lignifying Xylem of Angiosperms and Gymnosperms: Monolignol Oxidation
- Laboratory Evaluation of Natural Decay Resistance and Efficacy of CCA-Treated Rubberwood (Hevea brasiliensis Muell. Arg.)
- Inhibition of the Photodiscoloration of Wood by Butyrylation
- Lipophilic Extractives from Eucalyptus globulus Pulp during Kraft Cooking Followed by TCF and ECF Bleaching
- Determination of Monosaccharide Composition of Eucalyptus globulus Wood by FTIR Spectroscopy
- Acetyl Group Distribution in Acetylated Wood Investigated by Microautoradiography
- Solid State NMR Analysis of β-13C-Enriched Lignocellulosic Material During Light-Induced Yellowing
- N-Hydroxy Compounds as New Internal Standards for the 31P-NMR Determination of Lignin Hydroxy Functional Groups
- 19F NMR Spectroscopy for the Quantitative Analysis of Carbonyl Groups in Lignins
- Reactions of the β-Aryl Ether Lignin Model 1-(4-Hydroxy-3-Methoxyphenyl)-2-(2-Methoxyphenoxy)-1-Propanol on Heating in Aqueous Solution
- Structural Analysis of Residual and Technical Lignins by 1H-13C Correlation 2D NMR-Spectroscopy
- Experimental Determination of Stiffness Variation Across Growth Rings in Picea abies
- Determination of the Material Property Variations Across the Growth Ring of Softwood for Use in a Heterogeneous Drying Model Part 1. Capillary Pressure,Tracheid Model and Absolute Permeability
- Surface Area Determinations in Woodpulps by Humidity Adsorption
- A Simple Testing Method for the Measurement of the Water Vapour Transmission of Coated Wood Longitudinal and Tangential to Grain Direction
- Literature Reports
