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Steroid binding properties of the 2nd WHO International Standard for sex hormone-binding globulin

  • Markus Thaler , Carolin Müller , Alice Schlichtiger , Katharina Gründler , Melanie Moore and Peter B. Luppa EMAIL logo
Published/Copyright: February 24, 2011
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Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 49 Issue 5

Abstract

Background: Stocks of 95/560, the 1st International Standard for sex hormone-binding globulin (SHBG), are runn-ing out, and 08/266 has been prepared as a replacement. We compared the steroid binding capacities of 95/560 and 08/266.

Methods: 95/560 and 08/266 stored at –20°C, and accelerated thermal degradation samples of 08/266 were subjected to gelfiltration. Binding of the SHBG-containing fractions to a dihydrotestosterone derivative was then investigated using a surface plasmon resonance biosensor. The SHBG content of the gelfiltration fractions was determined with an immunoassay. Steroid binding capacity of each standard was then calculated as the mean of the ratio of biosensor binding/SHBG concentration. Affinity constants for the SHBG steroid interaction were calculated.

Results: Maximum achievable biomolecular interactions were lower for 95/560 than for the 08/266 preparations. 95/560 exhibited steroid binding capacity only half as high as any of the novel standards, as well as a lower affinity constant for the SHBG steroid interaction. No significant differences could be observed between the samples of 08/266 stored at different temperatures.

Conclusions: The steroid binding capacity of 08/266 is two times higher than that of 95/560. Steroid binding of lyophilized 08/266 is preserved at storage temperatures of up to 45°C for at least 6 months.

Keywords: biosensor; sex hormone-binding globulin; standard
Corresponding author: Prof. Dr. med. Peter B. Luppa, Institut für Klinische Chemie und Pathobiochemie, Klinikum rechts der Isar der Technischen Universität München, Ismaninger Str. 22, D-81675 München, Germany Phone: +49-89-4140-4759, Fax: +49-89-4140-4875
Received: 2010-9-7
Accepted: 2010-12-29
Published Online: 2011-02-24
Published in Print: 2011-05-01

©2011 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/CCLM.2011.146
Keywords for this article
biosensor; sex hormone-binding globulin; standard

Articles in the same Issue

  1. Editorials
  2. Laboratory testing and/or monitoring of the new oral anticoagulants/antithrombotics: for and against?
  3. Circulating macrocomplexes: old wine in new bottles?
  4. Review
  5. Laboratory assessment of new anticoagulants
  6. Minireviews
  7. Biomarkers in Alzheimer’s disease
  8. Analytical variability in sport hematology: its importance in an antidoping setting
  9. The plodding diagnosis of monogenic autoinflammatory diseases in childhood: from the clinical scenery to laboratory investigation
  10. Opinion Paper
  11. Collective opinion paper on findings of the 2010 convocation of experts on laboratory quality
  12. Genetics and Molecular Diagnostics
  13. Genetic variability of the fructosamine 3-kinase gene in diabetic patients
  14. Identification of Bcl-2/IgH fusion sequences using real-time PCR and chip-based microcapillary electrophoresis
  15. Performance evaluation of a multiplex assay for future use in biomarker discovery efforts to predict body composition
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  33. An increase in serum uric acid concentrations is associated with an increase in the Framingham risk score in Korean adults
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  35. False positive troponin result caused by a true macrotroponin
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