Article
Licensed
Unlicensed
Requires Authentication
High-performance metabolic marker assessment in breast cancer tissue by mass spectrometry
-
Published/Copyright:
December 14, 2010
Abstract
Background: The identification of reliable markers for diagnosis of breast cancer has been thoroughly addressed by metabolic profiling using nuclear magnetic resonance (NMR) spectroscopy or imaging. Several clear diagnostic indicators have emerged using either in vitro analysis of tissue extracts, ex vivo analysis of biopsies or in vivo direct spectral observations. Most of the breast cancer characteristic metabolites could be assayed by mass spectrometry (MS) to exploit the superior sensitivity of this technique and therefore reduce the traumatic impact of current biopsy procedures.
Methods: Following extraction, aqueous metabolite mixtures were obtained that were submitted to liquid-chromatography, electrospray-ionization, mass spectrometry (LC/ESI-MS) analysis to estimate the content of choline (Cho) and its phosphorylated derivatives, phosphocholine (PCho) and glycerophosphocholine (GPCho). The determinations were performed using 10 samples from breast tissue biopsies, surgical specimens and one single sample of a hepatic metastasis. In addition, some measurements were also repeated using high-resolution 1H NMR spectroscopy to complement the mass spectrometry results.
Results: The contents of Cho, PCho and GPCho in breast tissue extracts were estimated by LC/ESI-MS based on standard compound calibration curves. Sharply increased ratios of phosphorylated-to-unphosphorylated metabolites, PCho/ Cho and (PCho+GPCho)/Cho, were observed in all tumor samples, although without discrimination between benign and malignant lesions, contrary to samples from healthy individuals and from those with fibrocystic disease.
Conclusions: The assessment of breast cancer markers by LC/ESI-MS is feasible and diagnostically valuable. In addition to high sensitivity, the approach also shows a resolution advantage for assaying choline derivatives compared to NMR, and could complement the latter.
Keywords: breast cancer; breast tissue metabonomics; choline metabolites; mass spectrometry metabonomics; tumor marker detection by nuclear magnetic resonance and mass spectrometry
Received: 2010-2-12
Accepted: 2010-8-17
Published Online: 2010-12-14
Published in Print: 2011-02-01
©2011 by Walter de Gruyter Berlin New York
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Editorial
- Hepcidin: the main regulator of iron homeostasis
- Reviews
- Global trends in critical values practices and their harmonization
- Ischemia modified albumin changes – review and clinical implications
- Genetics and Molecular Diagnostics
- Decreased serum brain-derived neurotrophic factor (BDNF) is associated with post-stroke depression but not with BDNF gene Val66Met polymorphism
- A novel liquidchip platform for simultaneous detection of 70 alleles of DNA somatic mutations on EGFR, KRAS, BRAF and PIK3CA from formalin-fixed and paraffin-embedded slides containing tumor tissue
- General Clinical Chemistry and Laboratory Medicine
- Mass spectrometry measurement of plasma hepcidin for the prediction of iron overload
- Serum hepcidin-25 in comparison to biochemical markers and hematological indices for the differentiation of iron-restricted erythropoiesis
- The quality of the extra-analytical phase of laboratory practice in some developing European countries and Mexico – a multicentric study
- Impact of cryopreservation on serum concentration of matrix metalloproteinases (MMP)-7, TIMP-1, vascular growth factors (VEGF) and VEGF-R2 in Biobank samples
- Determinants of circulating asymmetric and symmetric dimethylarginines in patients evaluated for acute dyspnea
- Urinary stone composition in pediatric patients: a retrospective study of 205 cases
- Electrolyte-balanced heparin in blood gas syringes can introduce a significant bias in the measurement of positively charged electrolytes
- Full-scan mass spectral evidence for 3-epi-25-hydroxyvitamin D3 in serum of infants and adults
- Association of nucleated red blood cells in blood and arterial oxygen partial tension
- Validation and Outcome Studies
- Cystatin C is a reliable marker for estimation of glomerular filtration rate in renal transplantation: validation of a new turbidimetric assay using monospecific sheep antibodies
- Analytical validation of the Liaison Calcitonin_II-Gen (DiaSorin)
- A new tool in the field of in-vitro diagnosis of allergy: preliminary results in the comparison of ImmunoCAP© 250 with the ImmunoCAP© ISAC
- Rapid microwave digestion and microplate reading format method for urinary iodine determination
- Reference Values and Biological Variations
- Serum creatinine concentrations in male and female elite swimmers. Correlation with body mass index and evaluation of estimated glomerular filtration rate
- Biological variation of free β chorionic gonadotropin and pregnancy-associated plasma protein A in first trimester pregnancies
- Non-infectious conditions and gestational age influence C-reactive protein values in newborns during the first 3 days of life
- Cancer Diagnostics
- Interpretation of increments in serial tumour biomarker concentrations depends on the distance of the baseline concentration from the cut-off
- Performance of nuclear matrix protein 22 urine marker and voided urine cytology in the detection of urinary bladder tumors
- High-performance metabolic marker assessment in breast cancer tissue by mass spectrometry
- BRAF analysis by fine needle aspiration biopsy of thyroid nodules improves preoperative identification of papillary thyroid carcinoma and represents a prognostic factor. A mono-institutional experience
- Letters to the Editor
- Stat laboratory timeliness management according to clinician needs
- Stability of serum samples and hemolysis interference on the high sensitivity troponin T assay
- Falsely diminished results for insulin concentrations measured with the IMMULITE® 2000
- Neutrophil gelatinase-associated lipocalin (NGAL) determined in urine with the Abbott Architect or in plasma with the Biosite Triage? The laboratory's point of view
Articles in the same Issue
- Editorial
- Hepcidin: the main regulator of iron homeostasis
- Reviews
- Global trends in critical values practices and their harmonization
- Ischemia modified albumin changes – review and clinical implications
- Genetics and Molecular Diagnostics
- Decreased serum brain-derived neurotrophic factor (BDNF) is associated with post-stroke depression but not with BDNF gene Val66Met polymorphism
- A novel liquidchip platform for simultaneous detection of 70 alleles of DNA somatic mutations on EGFR, KRAS, BRAF and PIK3CA from formalin-fixed and paraffin-embedded slides containing tumor tissue
- General Clinical Chemistry and Laboratory Medicine
- Mass spectrometry measurement of plasma hepcidin for the prediction of iron overload
- Serum hepcidin-25 in comparison to biochemical markers and hematological indices for the differentiation of iron-restricted erythropoiesis
- The quality of the extra-analytical phase of laboratory practice in some developing European countries and Mexico – a multicentric study
- Impact of cryopreservation on serum concentration of matrix metalloproteinases (MMP)-7, TIMP-1, vascular growth factors (VEGF) and VEGF-R2 in Biobank samples
- Determinants of circulating asymmetric and symmetric dimethylarginines in patients evaluated for acute dyspnea
- Urinary stone composition in pediatric patients: a retrospective study of 205 cases
- Electrolyte-balanced heparin in blood gas syringes can introduce a significant bias in the measurement of positively charged electrolytes
- Full-scan mass spectral evidence for 3-epi-25-hydroxyvitamin D3 in serum of infants and adults
- Association of nucleated red blood cells in blood and arterial oxygen partial tension
- Validation and Outcome Studies
- Cystatin C is a reliable marker for estimation of glomerular filtration rate in renal transplantation: validation of a new turbidimetric assay using monospecific sheep antibodies
- Analytical validation of the Liaison Calcitonin_II-Gen (DiaSorin)
- A new tool in the field of in-vitro diagnosis of allergy: preliminary results in the comparison of ImmunoCAP© 250 with the ImmunoCAP© ISAC
- Rapid microwave digestion and microplate reading format method for urinary iodine determination
- Reference Values and Biological Variations
- Serum creatinine concentrations in male and female elite swimmers. Correlation with body mass index and evaluation of estimated glomerular filtration rate
- Biological variation of free β chorionic gonadotropin and pregnancy-associated plasma protein A in first trimester pregnancies
- Non-infectious conditions and gestational age influence C-reactive protein values in newborns during the first 3 days of life
- Cancer Diagnostics
- Interpretation of increments in serial tumour biomarker concentrations depends on the distance of the baseline concentration from the cut-off
- Performance of nuclear matrix protein 22 urine marker and voided urine cytology in the detection of urinary bladder tumors
- High-performance metabolic marker assessment in breast cancer tissue by mass spectrometry
- BRAF analysis by fine needle aspiration biopsy of thyroid nodules improves preoperative identification of papillary thyroid carcinoma and represents a prognostic factor. A mono-institutional experience
- Letters to the Editor
- Stat laboratory timeliness management according to clinician needs
- Stability of serum samples and hemolysis interference on the high sensitivity troponin T assay
- Falsely diminished results for insulin concentrations measured with the IMMULITE® 2000
- Neutrophil gelatinase-associated lipocalin (NGAL) determined in urine with the Abbott Architect or in plasma with the Biosite Triage? The laboratory's point of view
