Evolution of vitamin D binding protein concentration in sera from cardiac surgery patients is determined by triglyceridemia
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Marijn M. Speeckaert
Abstract
Background: Multiple human and animal studies have reviewed the (non-)actin scavenger functions of vitamin D binding protein (DBP). Recently, we demonstrated the partially lipid bound character of DBP. The purpose of the present study was to explore the link between actin, lipids and DBP in a cohort of patients undergoing cardiac surgery.
Methods: The interplay between DBP, actin and lipids was investigated in a cohort study of 35 Caucasian patients who underwent cardiac surgery. Total and actin-free DBP concentrations were assessed by immunonephelometry and ELISA. Total cholesterol, high-density lipoprotein (HDL) cholesterol, triglycerides, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, creatine kinase (-MB) and C-reactive protein (CRP) were measured using routine methods. Low-density lipoprotein cholesterol was calculated according to the Friedewald formula. The DBP phenotypes and the macromolecular bound DBP fractions were determined by polyacrylamide gel electrophoresis (PAGE) and confirmed by Western blotting.
Results: Using PAGE and Western blotting with an anti-human DBP polyclonal antibody, the actin-bound DBP complex was identified in serum. Following cardiac surgery, total serum DBP concentrations were characterized by a two phased course. This was paralleled by a similar pattern in serum total cholesterol, HDL-cholesterol and triglyceride concentrations. Good correlation was found between total and actin free serum DBP concentrations (r=0.69, p<0.0001). The serum actin free DBP/total DBP ratio remained stable throughout the study period. Although no significant correlation between cumulative CK-MB enzyme release and delta serum total DBP concentration was observed (p=NS), the latter value correlated significantly with delta serum triglyceride concentrations (r=0.37, p<0.05).
Conclusions: The lipid bound character is an underestimated property of DBP in the extracellular actin-scavenger system.
Clin Chem Lab Med 2010;48:1345–50.
©2010 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Editorial
- Lost in translation…wallowing in transformation?
- Reviews
- Coeliac disease – a diagnostic and therapeutic challenge
- Advancements in anemias related to chronic conditions
- Opinion Paper
- Translations of the “Evidence-Based Medicine” concept in different languages: is it time for international standardisation?
- Genetics and Molecular Diagnostics
- Rapid detection of glycogen storage disease type Ia by DNA microarray
- Transfer of myxovirus-protein-A mRNA assay for interferon-β bioactivity measurement in multiple sclerosis patients to routine laboratory practice. A 4-year experience
- General Clinical Chemistry and Laboratory Medicine
- Transferrin receptor expression on reticulocytes as a marker of iron status in dialyzed patients
- Quartz crystal microbalance-based biosensor for the detection of α-thalassemia 1 (SEA deletion)
- Retrospective analysis of 88,429 serum and urine glucose EQA results obtained from professional laboratories and medical offices participating in surveys organized by three European EQA centers between 1996 and 2007
- Liquid phase immunoassays utilizing magnetic markers and SQUID magnetometer
- L-Phenylalanine concentration in blood of phenylketonuria patients: a modified enzyme colorimetric assay compared with amino acid analysis, tandem mass spectrometry, and HPLC methods
- Amniotic fluid α-fetoprotein microheterogeneity in the prenatal diagnosis of congenital disorders of glycosylation type Ia
- Minor histocompatibility antigen HA-1 and HA-2 polymorphisms in Taiwan: frequency and application in hematopoietic stem cell transplantation
- Reference Values and Biological Variations
- Blood cell and iron status analytes of preterm and full-term infants from 20 weeks onwards during the first year of life
- Cancer Diagnostics
- Quantitative denaturing high performance liquid chromatography (Q-dHPLC) detection of APC long DNA in faeces from patients with colorectal cancer
- Rapid intraoperative parathyroid hormone assay in fine needle aspiration for differential diagnosis in thyroid and parathyroid surgery
- Serum albumin-bound proteomic signature for early detection and staging of hepatocarcinoma: sample variability and data classification
- Prevalence of atrophic gastritis in dyspeptic patients in Piedmont. A survey using the GastroPanel test
- Cardiovascular Diseses
- Diagnostic accuracy of B-type natriuretic hormone for congenital heart disease in the first month of life
- Carcinoembryonic antigen concentrations in patients with acute coronary syndrome
- Evolution of vitamin D binding protein concentration in sera from cardiac surgery patients is determined by triglyceridemia
- Letters to the Editor
- Effect of storage temperature or collection tube type on blood methylmalonic acid concentrations measured by gas chromatography-mass spectrometry
- Acute porphyrias may be overlooked in patients taking methenamine hippurate
- Reduced plasma apolipoprotein A-V concentrations in two lecithin:cholesterol acyltransferase deficient patients
- Salivary calcium: a risk indicator in periodontal disease
- S100B protein increases in human blood and urine during stressful activity
- Congress Abstracts
- Congress of Clinical Chemistry and Laboratory Medicine 7th Annual Conference of the German Society for Clinical Chemistry and Laboratory Medicine (DGKL) Mannheim, Germany, 29th September – 02nd October, 2010
- 9th National Congress of the Greek Society of Clinical Chemistry Athens, Greece, October 7 – 9, 2010
- 42nd National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology ROME MARRIOTT PARK HOTEL, Rome (Italy), 5 – 8 October 2010
