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Combined analysis of survivin autoantibody and carcinoembryonic antigen biomarkers for improved detection of colorectal cancer

  • Jinn-Shiun Chen , Kuei-Tien Chen , Wen-Chun Fan , Jau-Song Yu , Yu-Sun Chang and Err-Cheng Chan
Published/Copyright: February 24, 2010
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Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 48 Issue 5

Abstract

Background: Survivin is a member of the family of inhibitor of apoptosis proteins that is overexpressed in several human tumors. Previous studies have found that overexpression of survivin in cancer cells induces an antibody response.

Methods: We compared 232 serum samples from colorectal cancer (CRC) patients and 365 samples from healthy volunteers using an in vitro enzyme-linked immunosorbent assay to evaluate the survivin autoantibody response in patients.

Results: The sensitivity of the anti-survivin response from patients with CRC was 56.9%, and the specificity was 64.1%. When a cut-off value of 5.0 ng/mL was chosen for carcinoembryonic antigen (CEA) in these same serum samples, the values for sensitivity and specificity were 40.9% and 86.6%, respectively. Combined detection using survivin autoantibodies and CEA produced better sensitivity (51.3%) and specificity (89.9%) compared to the sensitivity of CEA (40.9%) and the specificities of the individual markers (64.1% and 86.6%, respectively). The area under a receiver operating characteristic curve was 0.617 for survivin autoantibodies, 0.630 for CEA and 0.694 for both markers together.

Conclusions: A positive association between autoantibodies against survivin and preoperative CEA concentrations in sera of patients with CRCs was established. Our results suggest that analysis of both parameters would assist in screening patients with CRC.

Clin Chem Lab Med 2010;48:719–25.

Keywords: carcinoembryonic antigen; colorectal cancer; enzyme-linked immunosorbent assay; survivin autoantibody
Corresponding authors: Dr. Jinn-Shiun Chen, Colorectal Section, Department of Surgery, Chang Gung Memorial Hospital, 5 Fu-Shin Street, Kweishan, Taoyuan, 333, Taiwan Phone: +886-3-3281200 (2101), Fax: +886-3-3278355, Dr. Err-Cheng Chan, Department of Medical Biotechnology and Laboratory Science, Chang Gung University, 259 Wen-Hua 1st Road, Kweishan, Taoyuan, Taiwan Phone: +886-3-2118800 (5220), Fax: +886-3-2118741,
Received: 2009-10-18
Accepted: 2009-12-28
Published Online: 2010-02-24
Published in Print: 2010-05-01

©2010 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/CCLM.2010.123
Keywords for this article
carcinoembryonic antigen; colorectal cancer; enzyme-linked immunosorbent assay; survivin autoantibody

Articles in the same Issue

  1. Editorials
  2. Laboratory medicine and natural disasters: are we ready for the challenge?
  3. Reference values: still a critical issue in laboratory medicine
  4. Review
  5. Contemporary platelet function testing
  6. Minireviews
  7. Disasters: where they find us
  8. Inter-laboratory variation in cerebrospinal fluid biomarkers for Alzheimer's disease: united we stand, divided we fall
  9. Opinion Paper
  10. Glycated hemoglobin (HbA1c): old dogmas, a new perspective?
  11. Guidelines and Recommendations
  12. IFCC reference procedures for measurement of the catalytic concentrations of enzymes: corrigendum, notes and useful advice
  13. Recommendations for the implementation of international standardization of glycated hemoglobin in Italy
  14. Genetics and Molecular Diagnostics
  15. UGT2B17 and SULT1A1 gene copy number variation (CNV) detection by LabChip microfluidic technology
  16. Genetic polymorphisms of CYP1A2, CYP3A4, CYP3A5, pregnane/steroid X receptor and constitutive androstane receptor in 207 healthy Spanish volunteers
  17. Automated genomic DNA extraction from saliva using the QIAxtractor
  18. General Clinical Chemistry and Laboratory Medicine
  19. A new statistical method for evaluating long-term analytical performance of laboratories applied to an external quality assessment scheme for flow cytometry
  20. Evaluation of the Becton-Dickinson rapid serum tube: does it provide a suitable alternative to lithium heparin plasma tubes?
  21. Immunoglobulin M antibodies to aquaporin-4 in neuromyelitis optica and related disorders
  22. Evaluation of the new body fluid mode on the Sysmex XE-5000 for counting leukocytes and erythrocytes in cerebrospinal fluid and other body fluids
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  24. Positive predictive value of serological diagnostic measures in celiac disease
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  31. Cancer Diagnostics
  32. Combined analysis of survivin autoantibody and carcinoembryonic antigen biomarkers for improved detection of colorectal cancer
  33. Oligoclonal bands and immunoglobulin isotype switch during monitoring of patients with multiple myeloma and autologous hematopoietic cell transplantation: a 16-year experience
  34. Letters to the Editor
  35. Methodological weakness in using correlation coefficients for assessing the interchangeability of analyte data between samples collected under different sampling conditions – the example of matrix metalloproteinase 9 determined in serum and plasma samples
  36. Intralipid causes falsely increased glucose concentrations with the Hemocue glucose analyzer
  37. Serum cholesterol: required and actual bias values
  38. Abstracts
  39. ISMD2010 Eighth International Symposium on Molecular Diagnostics
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