Artikel
Lizenziert
Nicht lizenziert
Erfordert eine Authentifizierung
Expression of lineage markers using real-time quantitative polymerase chain reaction (RT-qPCR) in normal and in leukemia bone marrow
-
Pascale Saussoy
Veröffentlicht/Copyright:
1. April 2009
Abstract
Background: The study of lineage markers by real-time quantitative polymerase chain reaction (RT-qPCR) at diagnosis enables differentiation between acute myeloblastic leukemia, B- or T-lineage acute lymphoblastic leukemia, without cell sorting. Our objective was to assess the relationship between protein expression and the amount of lineage marker mRNA in acute leukemia samples and to determine whether four lineage markers could be used to differentiate between normal and acute leukemia bone marrow (BM) without cell sorting.
Methods: Quantification of the mRNA of CD19, CD79a, CD3e, and myeloperoxidase was performed by RT-qPCR on 130 acute leukemia BM samples at diagnosis and on 20 BM samples from healthy donors, without cell sorting. Immunophenotyping of leukemia samples was performed after manual gating around the blastic population.
Results: Reference values for the four lineage markers were established by RT-qPCR for normal BM. The mRNA expression levels of these four lineage markers allowed the distinction between normal samples and 100% of acute leukemia samples.
Conclusions: With 92% congruence for protein expression and amount of mRNA in acute leukemias, these four lineage markers, essential for diagnosis and subclassification of acute leukemias by flow cytometry, also represent excellent candidate genes when using RT-qPCR technology as a diagnostic tool for molecular cancer class prediction.
Clin Chem Lab Med 2009;47:419–26.
Received: 2008-9-23
Accepted: 2009-1-5
Published Online: 2009-04-01
Published in Print: 2009-04-01
©2009 by Walter de Gruyter Berlin New York
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Artikel in diesem Heft
- Minireview
- Risk loci for type 2 diabetes – Quo vadis?
- Genetics and Molecular Diagnostics
- A common variant in the FTO gene is associated with body mass index in males and postmenopausal females but not in premenopausal females. Czech post-MONICA and 3PMFs studies
- Genotype distribution of estrogen receptor α polymorphisms in pregnant women from healthy and preeclampsia populations and its relation to blood pressure levels
- Do common genetic variants in endotoxin signaling pathway contribute to predisposition to alcoholic liver cirrhosis?
- The selection and application of ssDNA aptamers against MPT64 protein in Mycobacterium tuberculosis
- Stromal cell-derived factor-1 but not its receptor, CXCR4, gene variants increase susceptibility and pathological development of hepatocellular carcinoma
- Expression of lineage markers using real-time quantitative polymerase chain reaction (RT-qPCR) in normal and in leukemia bone marrow
- Translation termination and protein folding pathway genes are not correlated in gastric cancer
- General Clincial Chemistry and Laboratory Medicine
- Novel serum paraoxonase activity assays are associated with coronary artery disease
- Elevated erythrocyte carbonic anhydrase activity is a novel clinical marker in hyperventilation syndrome
- Elevation of the glycoxidation product Nε-(carboxymethyl)lysine in patients presenting with acute myocardial infarction
- Lipoprotein(a) level and its association with tumor stage in male patients with primary lung cancer
- A high-performance liquid chromatography method for the determination of carbamazepine and carbamazepine-10,11-epoxide and its comparison with chemiluminescent immunoassay
- Reference Values and Biological Variations
- Minimizing the effects of multicollinearity in the polynomial regression of age relationships and sex differences in serum levels of pregnenolone sulfate in healthy subjects
- Determination of physiological plasma pentraxin 3 (PTX3) levels in healthy populations
- Validation and Outcome Studies
- Analytical precision of the Urolizer® for the determination of the BONN-Risk-Index (BRI) for calcium oxalate urolithiasis and evaluation of the influence of 24-h urine storage at moderate temperatures on BRI
- Determination of nitrotyrosine concentrations in plasma samples of diabetes mellitus patients by four different immunoassays leads to contradictive results and disqualifies the majority of the tests
- Estimation of trueness of measurement results obtained in external quality assessment
- Letters to the Editor
- A new PCR-RFLP assay for –1123 G>C polymorphism in the PTPN22 gene: allele and genotype frequencies in a western Mexican population
- Proposed classification of various limit values (guide values) used in assisting the interpretation of quantitative laboratory test results
- Case report: Over-substitution of thyroxine due to interference in serum thyroid-stimulating hormone measurement
- Rv2629 191A/C nucleotide change is not associated with rifampicin resistance in Mycobacterium tuberculosis
Schlagwörter für diesen Artikel
bone marrow;
immunophenotyping;
lineage marker;
real-time PCR;
reference values
Artikel in diesem Heft
- Minireview
- Risk loci for type 2 diabetes – Quo vadis?
- Genetics and Molecular Diagnostics
- A common variant in the FTO gene is associated with body mass index in males and postmenopausal females but not in premenopausal females. Czech post-MONICA and 3PMFs studies
- Genotype distribution of estrogen receptor α polymorphisms in pregnant women from healthy and preeclampsia populations and its relation to blood pressure levels
- Do common genetic variants in endotoxin signaling pathway contribute to predisposition to alcoholic liver cirrhosis?
- The selection and application of ssDNA aptamers against MPT64 protein in Mycobacterium tuberculosis
- Stromal cell-derived factor-1 but not its receptor, CXCR4, gene variants increase susceptibility and pathological development of hepatocellular carcinoma
- Expression of lineage markers using real-time quantitative polymerase chain reaction (RT-qPCR) in normal and in leukemia bone marrow
- Translation termination and protein folding pathway genes are not correlated in gastric cancer
- General Clincial Chemistry and Laboratory Medicine
- Novel serum paraoxonase activity assays are associated with coronary artery disease
- Elevated erythrocyte carbonic anhydrase activity is a novel clinical marker in hyperventilation syndrome
- Elevation of the glycoxidation product Nε-(carboxymethyl)lysine in patients presenting with acute myocardial infarction
- Lipoprotein(a) level and its association with tumor stage in male patients with primary lung cancer
- A high-performance liquid chromatography method for the determination of carbamazepine and carbamazepine-10,11-epoxide and its comparison with chemiluminescent immunoassay
- Reference Values and Biological Variations
- Minimizing the effects of multicollinearity in the polynomial regression of age relationships and sex differences in serum levels of pregnenolone sulfate in healthy subjects
- Determination of physiological plasma pentraxin 3 (PTX3) levels in healthy populations
- Validation and Outcome Studies
- Analytical precision of the Urolizer® for the determination of the BONN-Risk-Index (BRI) for calcium oxalate urolithiasis and evaluation of the influence of 24-h urine storage at moderate temperatures on BRI
- Determination of nitrotyrosine concentrations in plasma samples of diabetes mellitus patients by four different immunoassays leads to contradictive results and disqualifies the majority of the tests
- Estimation of trueness of measurement results obtained in external quality assessment
- Letters to the Editor
- A new PCR-RFLP assay for –1123 G>C polymorphism in the PTPN22 gene: allele and genotype frequencies in a western Mexican population
- Proposed classification of various limit values (guide values) used in assisting the interpretation of quantitative laboratory test results
- Case report: Over-substitution of thyroxine due to interference in serum thyroid-stimulating hormone measurement
- Rv2629 191A/C nucleotide change is not associated with rifampicin resistance in Mycobacterium tuberculosis
