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High concentrations of haptocorrin interfere with routine measurement of cobalamins in human serum and milk. A problem and its solution
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Dorte L. Lildballe
Published/Copyright:
February 1, 2009
Abstract
Background: Human milk and occasional serum samples contain high concentrations of unsaturated haptocorrin, which influence accurate measurement of cobalamins.
Methods: Cobalamins in serum samples spiked with increasing amounts of unsaturated haptocorrin were measured employing the Centaur, Cobas and Architect analysers. Cobinamide-coated EAH sepharose was employed for pretreatment of the samples. Human milk samples were collected from 24 healthy mothers. Haptocorrin was measured by ELISA.
Results: The measured concentration of cobalamins either increased (Centaur analyser) or decreased (Architect, Cobas analysers) significantly for haptocorrin >10 nM, and was 220%, 52% or 45% of the expected values in a serum sample containing 50 nM haptocorrin. Following pretreatment with cobinamide-sepharose, the expected cobalamin concentration was obtained (Centaur). The milk samples contained 4.5–180 nM haptocorrin. In samples containing >10 nM haptocorrin (n=19), the median concentration of cobalamins decreased from 1.3 nM to 0.67 nM after pretreatment with cobinamide-sepharose.
Conclusions: Haptocorrin in concentrations above 10 nM influences measurement of cobalamins giving rise to falsely elevated or decreased results. Removal of unsaturated haptocorrin by pretreatment with cobinamide-sepharose solves the problem.
Clin Chem Lab Med 2009;47:182–7.
Received: 2008-10-6
Accepted: 2008-11-21
Published Online: 2009-02-01
Published in Print: 2009-02-01
©2009 by Walter de Gruyter Berlin New York
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- Editorial
- 50th Anniversary of SBCC/BVKC: “International Symposium Brussels 2008: New Trends in Clinical Biology. Emerging Technologies and their Impact on Laboratory Medicine and Management”
- Reviews
- Vitamin D: current status and perspectives
- Circulating oxidized low-density lipoprotein: a biomarker of atherosclerosis and cardiovascular risk?
- New European guidelines for cardiovascular disease prevention in clinical practice
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