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Performance of free β-human chorionic gonadotrophin (free β-hCG) and pregnancy associated plasma protein-A (PAPP-A) analysis between Delfia Xpress and AutoDelfia systems in The Netherlands
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Ingeborg H. Linskens
Published/Copyright:
February 1, 2009
Abstract
Background: The VU University Medical Center (VUmc) was the first hospital in the Netherlands to introduce the Delfia Xpress for the analysis of free β-human chorionic gonadotrophin (β-hCG) and pregnancy associated plasma protein-A (PAPP-A) in the first trimester screening program for Down syndrome. Since then, others have implemented this system. In this study, we tested the equality of measurements for free β-hCG and PAPP-A between Delfia Xpress systems and one AutoDelfia system.
Methods: A total of 40 serum samples were aliquoted and stored at –20°C. Samples were analyzed by six Delfia Xpress systems and one AutoDelfia system over a time period of 2 years.
Results: The relationships between free β-hCG and PAPP-A were excellent for the different Delfia Xpress systems (r>0.99, p<0.0001). For PAPP-A, the agreement between the main system at VUmc and five other systems was linear with slopes between 0.99 and 1.06. Similarly, agreement for free β-hCG was linear with slopes between 0.99 and 1.09. Likewise, agreement for PAPP-A and free β-hCG was excellent for the AutoDelfia vs. the main Delfia Xpress at the VUmc (r>0.99, p<0.0001). For both PAPP-A and free β-hCG, the relationships were linear with slopes of 1.08 and 1.07.
Conclusions: We demonstrate an excellent agreement for the analysis of PAPP-A and free β-hCG between Delfia Xpress systems and one AutoDelfia system.
Clin Chem Lab Med 2009;47:222–6.
Keywords: AutoDelfia; Delfia Xpress; first trimester Down syndrome screening; free β-human chorionic gonadotrophin; pregnancy associated plasma protein-A
Received: 2008-7-1
Accepted: 2008-11-6
Published Online: 2009-02-01
Published in Print: 2009-02-01
©2009 by Walter de Gruyter Berlin New York
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Keywords for this article
AutoDelfia;
Delfia Xpress;
first trimester Down syndrome screening;
free β-human chorionic gonadotrophin;
pregnancy associated plasma protein-A
Articles in the same Issue
- Editorial
- 50th Anniversary of SBCC/BVKC: “International Symposium Brussels 2008: New Trends in Clinical Biology. Emerging Technologies and their Impact on Laboratory Medicine and Management”
- Reviews
- Vitamin D: current status and perspectives
- Circulating oxidized low-density lipoprotein: a biomarker of atherosclerosis and cardiovascular risk?
- New European guidelines for cardiovascular disease prevention in clinical practice
- Review
- Causes, consequences, detection, and prevention of identification errors in laboratory diagnostics
- Genetics and Molecular Diagnostics
- The PCSK9 gene E670G polymorphism affects low-density lipoprotein cholesterol levels but is not a risk factor for coronary artery disease in ethnic Chinese in Taiwan
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- Methodology for monitoring cytomegalovirus infection after renal transplantation
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