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Evaluation of a new Sebia isoelectrofocusing kit for α1-antitrypsin phenotyping with the Hydrasys® System
-
Farid Zerimech
Veröffentlicht/Copyright:
13. Dezember 2007
Abstract
Background: Laboratory evaluation of α1-antitrypsin (A1AT) deficiency is generally performed by determination of A1AT concentrations and identification of specific allelic variants by phenotyping. For this purpose, we evaluated a new Hydragel 18 A1AT Isofocusing® kit on the semi-automatic Hydrasys® System (Sebia) for the determination of A1AT phenotypes by isoelectrofocusing on ready-to-use agarose gels with specific immunological detection.
Methods: Serum samples from 66 patients were analysed with this new kit in comparison with the conventional and manually performed isoelectrofocusing method on polyacrylamide gels with Coomassie Blue staining.
Results: A1AT phenotypes showed comparable iso-electrofocusing patterns in both systems. The good within-gel reproducibility of this kit was demonstrated using two normal serum samples (M1 and M1M2 phenotypes) and six pathological serum samples with different phenotypes (MS, SS, SZ, MZ, ZZ). A sensitivity study was undertaken by performing serial dilutions on a serum with a ZZ phenotype containing 0.27 g/L A1AT. The detection limit was 0.050 g/L.
Conclusions: This new method is highly specific, rapid and simple to perform. It improves identification of not only the most common but also various rare A1AT phenotypes. It appears to be suitable for routine analysis and screening applications in a clinical laboratory setting.
Clin Chem Lab Med 2008;46:260–3.
Received: 2007-7-9
Accepted: 2007-10-2
Published Online: 2007-12-13
Published in Print: 2008-02-01
©2008 by Walter de Gruyter Berlin New York
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Schlagwörter für diesen Artikel
α1-antitrypsin deficiency;
agarose gel;
Hydrasys®;
isoelectrofocusing
Artikel in diesem Heft
- Opinion Papers
- The double whammy of endogenous insulin antibodies in non-diabetic subjects
- ‘Likelihood-ratio’ and ‘odds’ applied to monitoring of patients as a supplement to ‘reference change value’ (RCV)
- Genetics and Molecular Diagnostics
- A family with multiple mutations and sequence variations in the α- and β-globin gene clusters
- CTLA-4 polymorphism 49A–G is associated with placental abruption and preeclampsia in Finnish women
- Rare TA repeats in promoter TATA box of the UDP glucuronosyltranferase (UGT1A1) gene in Croatian subjects
- Association of high sensitive C-reactive protein with apolipoprotein E polymorphism in children and young adults: The Cardiovascular Risk in Young Finns Study
- Relationship between thiolactonase activity and hyperhomocysteinemia according to MTHFR gene polymorphism in Tunisian Behçet's disease patients
- A rapid and sensitive method to detect specific human lymphocyte antigen (HLA) class II alleles associated with celiac disease
- General Clinical Chemistry and Laboratory Medicine
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- Long-term prognostic value of midregional pro-adrenomedullin and C-terminal pro-endothelin-1 in patients with acute myocardial infarction
- Changes in serum fetuin-A and inflammatory markers levels in end-stage renal disease (ESRD): effect of a single session haemodialysis
- Modeling the variability of creatinine measurements improves estimates of the glomerular filtration rate
- Reference Values
- Age-related changes of plasma glycosaminoglycans
- Stability of heparin blood samples during transport based on defined pre-analytical quality goals
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