Recommendation for measuring and reporting chloride by ISEs in undiluted serum, plasma or blood: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC): IFCC Scientific Division, Committee on Point of Care Testing and Working Group on Selective Electrodes

Mohammed C. Ben Rayana; Robert W. Burnett; Arthur K. Covington; Paul D'Orazio; Niels Fogh-Andersen; Ellis Jacobs; Ritu Kataky; Wolf R. Külpmann; Katsuhiko Kuwa; Lasse Larsson; Andrzej Lewenstam; Anton H.J. Maas; Gerhard Mager; Jerzy W. Naskalski; Anthony O. Okorodudu; Christoph Ritter; Andrew St John

doi:10.1515/CCLM.2006.060

Article

Recommendation for measuring and reporting chloride by ISEs in undiluted serum, plasma or blood: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC): IFCC Scientific Division, Committee on Point of Care Testing and Working Group on Selective Electrodes

Mohammed C. Ben Rayana , Robert W. Burnett , Arthur K. Covington , Paul D'Orazio , Niels Fogh-Andersen , Ellis Jacobs , Ritu Kataky , Wolf R. Külpmann , Katsuhiko Kuwa , Lasse Larsson , Andrzej Lewenstam , Anton H.J. Maas , Gerhard Mager , Jerzy W. Naskalski , Anthony O. Okorodudu , Christoph Ritter and Andrew St John

Published/Copyright: September 21, 2011

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From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 44 Issue 3

Abstract

The proposed recommendation for measuring and reporting chloride in undiluted plasma or blood by ion-selective electrodes (ISEs) will provide results that are identical to chloride concentrations measured by coulometry for standardized normal plasma or blood samples. It is applicable to all current ISEs dedicated to chloride measurement in undiluted samples that meet the requirements. However, in samples with reduced water concentration, results by coulometry are lower than by ion-selective electrode due to volume displacement. The quantity measured by this standardized ISE procedure is called the ionized chloride concentration. It may be clinically more relevant than the chloride concentration as determined by coulometry, photometry or by ISE after dilution of the sample.

Keywords: blood; chloride (ionized and total); coulometry; ion-selective electrode; plasma; standardization

Corresponding author: Prof. Dr. W.R. Külpmann, Klinische Chemie, Medizinische Hochschule Hannover, Carl-Neuberg-Str, 1, 30625 Hannover, Germany Phone: +49-5115326613, Fax: +49-5115328614, kuelpmann.wolf@mh-hannover.de

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Published Online: 2011-9-21

Published in Print: 2006-3-1

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Articles in the same Issue

https://doi.org/10.1515/CCLM.2006.060

Keywords for this article

blood; chloride (ionized and total); coulometry; ion-selective electrode; plasma; standardization