Comparison of the TaqMan and LightCycler systems in pharmacogenetic testing: evaluation of CYP2C9*2/*3 polymorphisms
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Mario Toriello
Abstract
Background: Pharmacogenetic testing for drug-metabolizing enzymes is not yet widely used in clinical practice.
Methods: In an attempt to facilitate the application of this procedure, we have compared two real-time PCR-based methods, the TaqMan® and the LightCycler™ for the pharmacogenetic evaluation of CYP2C9*2/*3 polymorphisms.
Results and Conclusion: Both procedures are suitable for pharmacogenetic studies. The TaqMan procedure was less expensive in terms of cost per sample, but the TaqMan apparatus is more expensive than the LightCycler apparatus.
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©2006 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Recent advances in physiological calcium homeostasis
- Associations of apolipoprotein E exon 4 and lipoprotein lipase S447X polymorphisms with acute ischemic stroke and myocardial infarction
- Association of angiotensin II type 1 receptor gene polymorphism with carotid atherosclerosis
- Comparison of the TaqMan and LightCycler systems in pharmacogenetic testing: evaluation of CYP2C9*2/*3 polymorphisms
- Urinary cystatin C as a specific marker of tubular dysfunction
- Time-resolved fluorimetric immunoassay of calprotectin: technical and clinical aspects in diagnosis of inflammatory bowel diseases
- Direct and fast determination of antiretroviral drugs by automated online solid-phase extraction-liquid chromatography-tandem mass spectrometry in human plasma
- Markers of oxidative stress in children with Down syndrome
- Influence of hemolysis on routine clinical chemistry testing
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- Comparison of the lipid profile and lipoprotein(a) between sedentary and highly trained subjects
- Clinical outcome estimates based on treatment target limits of laboratory tests: proposal for a plot visualizing effects and differences of medical target setting exemplified by glycemic control in diabetes
- Metrological traceability of values for catalytic concentration of enzymes assigned to a calibration material
- Evaluation of a new Sebia kit for analysis of hemoglobin fractions and variants on the Capillarys® system
- Recommendation for measuring and reporting chloride by ISEs in undiluted serum, plasma or blood: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC): IFCC Scientific Division, Committee on Point of Care Testing and Working Group on Selective Electrodes
- Diluent Multiassay for the MODULAR ANALYTICS E170 does not improve TSH dilutions compared to Diluent Universal
