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Analytical performance of a new two-step ADVIA Centaur® estradiol immunoassay during ovarian stimulation

  • Catherine Massart , Jacqueline Gibassier , Marie-Christine Laurent and Dominique Le Lannou
Published/Copyright: September 21, 2011
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 44 Issue 1

Abstract

Measurement of estradiol is useful in women undergoing ovarian stimulation for in vitro fertilization and embryo transfer (IVF-ET). The analytical performance of a new two-step estradiol assay (ADVIA Centaur® estradiol-6 III from Bayer Diagnostics) was evaluated in 41 sera from 11 women undergoing ovarian stimulation. The results were compared to those obtained with two radioimmunoassays (RIAs; RIA Estradiol Immunotech IM 1663 from Beckmann Coulter and Coat-A-Count Estradiol from Diagnostic Products Corporation) and with one chemiluminescent immunoassay (CLIA; ADVIA Centaur® estradiol-6). The ADVIA Centaur® estradiol-6 III assay was the most sensitive assay, with a functional sensitivity of 55pmol/L. Within- and between-run coefficients of variation calculated for the new ADVIA Centaur® assay ranged from 3.3% to 9%, which was better than the precision obtained for the other assays. A dilution test showed serum interferences when estradiol was measured in non-diluted samples. No statistical difference was observed between the estradiol results obtained in diluted sera with the new two-step ADVIA Centaur® assay and those measured with the Immunotech RIA and the other CLIA. In conclusion, this new, two-step estradiol assay performed on the ADVIA Centaur® system displays suitable sensitivity, precision and intermethod agreement with the Immunotech RIA for the measurement of serum estradiol concentrations in women undergoing ovarian stimulation and IVF-ET. For correct linearity, estradiol measurement should be performed on diluted samples.

Keywords: estradiol; immunoassay; ovarian stimulation; sensitivity
Corresponding author: Catherine Massart, Laboratoire de Génétique Moléculaire et Hormonologie, CHU de Pontchaillou, rue Le Guilloux, 35043 Rennes, France Fax: +33-299-284-145,

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Received: 2005-8-18
Accepted: 2005-10-18
Published Online: 2011-9-21
Published in Print: 2006-1-1

©2006 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/CCLM.2006.020
Keywords for this article
estradiol; immunoassay; ovarian stimulation; sensitivity

Articles in the same Issue

  1. Where does the evidence come from?
  2. NASBA: a novel approach to assess hormonal receptors and ERBB2 status in breast cancer
  3. Plasma cell-free DNA as an indicator of severity of injury in burn patients
  4. Bivariate statistical approach to evaluate laboratory performance by analysis of standard curves in an External Quality Assurance program for quantitative assays based on real-time PCR with Taq-Man™ probes
  5. Erythrocyte membrane acetylcholinesterase activity in subjects with MTHFR 677C→T genotype
  6. Plasma levels of tumor M2-pyruvate kinase should not be used as a tumor marker for hematological malignancies and solid tumors
  7. APO A-V–1131T→C polymorphism frequency and its association with morbidity in a Brazilian elderly population
  8. Association study between fibronectin and coronary heart disease
  9. Serum calcium and phosphorus associate with the occurrence and severity of angiographically documented coronary heart disease, possibly through correlation with atherogenic (apo)lipoproteins
  10. Urinary calcium excretion in severe preeclampsia and eclampsia
  11. In vitro re-mineralization of demineralized bone matrix in human serum
  12. Measurement of serum amyloid A1 (SAA1), a major isotype of acute phase SAA
  13. Disturbed lipoprotein composition in non-dialyzed, hemodialysis, continuous ambulatory peritoneal dialysis and post-transplant patients with chronic renal failure
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  18. Improvement in glycemic control over 11 years in patients monitored for diabetes in one county
  19. The clinical usefulness of glucose tolerance testing in gestational diabetes to predict early postpartum diabetes mellitus
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  21. EC4 European Syllabus for Post-Graduate Training in Clinical Chemistry and Laboratory Medicine: version 3 – 2005
  22. POX-Act assay and d-ROMs test – what are the facts?
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