Physiological matrix metalloproteinase concentrations in serum during childhood and adolescence, using Luminex® Multiplex technology

Kathryn M. Thrailkill; Cindy S. Moreau; Gael Cockrell; Pippa Simpson; Rajiv Goel; Paula North; John L. Fowlkes; Robert C. Bunn

doi:10.1515/CCLM.2005.238

Article

Physiological matrix metalloproteinase concentrations in serum during childhood and adolescence, using Luminex^® Multiplex technology

Kathryn M. Thrailkill , Cindy S. Moreau , Gael Cockrell , Pippa Simpson , Rajiv Goel , Paula North , John L. Fowlkes and Robert C. Bunn

Published/Copyright: September 21, 2011

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From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 43 Issue 12

Abstract

Matrix metalloproteinases are a family of zinc-dependent proteinases which are involved in the breakdown and remodeling of extracellular matrix. As children grow and adolescents reach pubescence, their bodies undergo changes that require age-related morphogenesis of the extracellular matrix, possibly requiring unique patterns of matrix metalloproteinase (MMP) expression during periods of rapid tissue growth (i.e., childhood) or accelerated tissue remodeling and expansion (i.e., adolescence). Therefore, we have characterized age-specific and gender-specific differences in circulating concentrations of MMPs (specifically MMP-1, -2, -3, -8 and -9) in 189 serum samples obtained from healthy subjects, aged 2–18 years. MMP concentrations were measured using Fluorokine^® MultiAnalyte Profiling kits and a Luminex^® Bioanalyzer, as well as by commercial ELISA. Serum levels of MMP-1, -2, -3, -8, and -9 in healthy pediatric subjects represent log-normal distributions. MMP-2 was significantly negatively correlated with age (r=−0.29; p<0.001), while MMP-3 was significantly positively correlated with age (r=0.38; p<0.001). Although plasma, not serum, is considered the appropriate blood sample for measurement of MMP-8 and -9, serum levels of MMP-8 and -9 were also found to be highly positively correlated with each other (r=0.76; p<0.01). MMP results obtained by Fluorokine^® MultiAnalyte Profiling methods correlated well with conventional ELISA methods and use of this technology provided several advantages over ELISA.

Keywords: collagenase; endopeptidases; gelatinase; particle-based flow cytometry; puberty; stromelysin

Corresponding author: Kathryn M. Thrailkill, MD, Arkansas Children's Hospital, 800 Marshall St., Slot 512-6, Little Rock, AR 72202, USA Phone: +1-501-364-1430, Fax: +1-501-364-6299, thrailkillkathrynm@uams.edu

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Received: 2005-7-21

Accepted: 2005-10-5

Published Online: 2011-9-21

Published in Print: 2005-12-1

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Articles in the same Issue

https://doi.org/10.1515/CCLM.2005.238

Keywords for this article

collagenase; endopeptidases; gelatinase; particle-based flow cytometry; puberty; stromelysin