Do we measure bilirubin correctly anno 2005?
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Joke J. Apperloo
Abstract
We observed 30% discrepancy between liquid chemistry and dry chemistry analysers for the determination of total bilirubin in human adult serum samples, which were consistent with a 20% overestimation and 10% underestimation relative to a Jendrassik-Grof reference method, respectively. In contrast, standard reference material SRM916, which was recently recommended as being the most suitable material for attaining interlaboratory agreement, shows very good agreement on both types of analysers, as well as close to 100% recovery with respect to the reference method. We show that the liquid vs. dry bilirubin discrepancies seem to originate in the presence of either conjugated or δ-bilirubin. Our observations make it clear that good interlaboratory (or inter-analyser) agreement between bilirubin reference materials does not guarantee the same for bilirubin concentrations in human serum samples.
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© by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Second Santorini Conference “From Human Genetic Variations to Prediction of Risks and Responses to Drugs and to the Environment”
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- Understanding hyperlipidemia and atherosclerosis: lessons from genetically modified apoe and ldlr mice
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- Both core and terminal glycosylation alter epitope expression in thyrotropin and introduce discordances in hormone measurements
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