β-Carotene stimulates chemotaxis of human endothelial progenitor cells
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Beata Kieć-Wilk
Abstract
Angiogenesis is a crucial process in tissue remodeling during growth, both in the embryo and the adult. In our study we concentrated on the direct effect of β-carotene on human umbilical cord originating from endothelial progenitor cells (EPCs). β-Carotene uptake by EPCs was measured using a HPLC method. The determination of cell surface antigens was performed by flow cytometry. The effect on cell proliferation was estimated by measuring bromo-deoxyuridine incorporation. The influence on the formation of a tubular-like structure was investigated in a 3D assay in matrigel. Quantitative gene expression was estimated using real-time PCR. We demonstrated that β-carotene in the physiological range of concentrations found in human blood is a potent activator of EPC chemotaxis, which is accompanied by a change in the expression of genes mediating cell adhesion and homing, but does not activate the final markers of endothelial differentiation. This study points to the prochemotactic and homing activity of β-carotene in undifferentiated endothelial cell progenitors for the first time, which may suggest a potential role of this carotenoid in progenitor cell therapy aimed at angiogenesis and tissue repair.
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Articles in the same Issue
- Second Santorini Conference “From Human Genetic Variations to Prediction of Risks and Responses to Drugs and to the Environment”
- Expressed genome molecular signatures of heart failure
- Understanding hyperlipidemia and atherosclerosis: lessons from genetically modified apoe and ldlr mice
- Alcohol and gene interactions
- β-Carotene stimulates chemotaxis of human endothelial progenitor cells
- Effect of interferon-γ, interleukin-10, lipopolysaccharide and tumor necrosis factor-α on chitotriosidase synthesis in human macrophages
- Two immunochemical assays to measure advanced glycation end-products in serum from dialysis patients
- Apolipoprotein E haplotyping by denaturing high-performance liquid chromatography
- Both core and terminal glycosylation alter epitope expression in thyrotropin and introduce discordances in hormone measurements
- Do we measure bilirubin correctly anno 2005?
- Differences in mortality on the basis of laboratory parameters in an unselected population at the Emergency Department
- An Italian program of external quality control for quantitative assays based on real-time PCR with Taq-Man™ probes
- A reference material for traceability of aspartate aminotransferase (AST) results
- Harmonization of the Bayer ADVIA Centaur and Abbott AxSYM automated B-type natriuretic peptide assay in patients on hemodialysis
- Multicenter evaluation of the analytical and clinical performance of the Elecsys ® S100 immunoassay in patients with malignant melanoma
- Guidelines for sampling, measuring and reporting ionized magnesium in undiluted serum, plasma or blood: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC): IFCC Scientific Division, Committee on Point of Care Testing
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