Home Medicine Interference of dextran in biuret-type assays of serum proteins
Article
Licensed
Unlicensed Requires Authentication

Interference of dextran in biuret-type assays of serum proteins

  • Joris R. Delanghe , Nicole Hamers , Youri E. Taes and Jean-Claude Libeer
Published/Copyright: September 21, 2011

Abstract

Dextran interference in biuret-type assays of total serum proteins was investigated in a Belgian National External Quality Assurance Survey with 256 participants. In vitro supplementation of therapeutic (10% Gentran 70 ®) dextran concentrations showed a broadly varying (from 0 to 20%) negative interference. The analytical interference was found to depend on both the sodium hydroxide and tartrate concentrations in the reagent formulation. The dry chemistry biuret method was not affected by the dextran interference. In a number of cases, the effects observed may be of clinical importance. Both clinicians and laboratory staff should be aware of the persistence of this analytical problem.


Corresponding author: Prof. Dr. Joris Delanghe, Laboratory Clinical Chemistry 2P8, University Hospital Ghent, De Pintelaan 185, 9000 Ghent, Belgium Phone: +32-9-2402956, Fax: +32-9-2404985,

References

1 Nearman HS, Herman ML. Toxic effects of colloids in the intensive care unit. Crit Care Clin 1991; 7: 713–23. 10.1016/S0749-0704(18)30302-6Search in Google Scholar

2 Brecher ME, Owen HG, Bandarenko N. Alternatives to albumin: starch replacement for plasma exchange. J Clin Apheresis 1997; 12: 146–53. 10.1002/(SICI)1098-1101(1997)12:3<146::AID-JCA8>3.0.CO;2-ASearch in Google Scholar

3 Weber JA, Van Zanten AP. Interference of plasma expanders in determination of total protein. Clin Chem 1989; 35: 2143–4. 10.1093/clinchem/35.10.2143Search in Google Scholar

4 Gornall AG, Bardawill J, David MM. Determination of serum proteins by means of the biuret reaction. J Biol Chem 1949; 177: 751–66. 10.1016/S0021-9258(18)57021-6Search in Google Scholar

5 Sykes E, Grzych C, Epstein E, Kiechle FL. Dextran interference in biuret-based assays for serum proteins. Clin Chem 1987; 33: 1073–4. 10.1093/clinchem/33.6.1073Search in Google Scholar

6 Barnes DB, Pierce GF, Lichti D, Landt M, Koenig J, Chan KM. Effects of dextran on five biuret-based procedures for total protein in serum. Clin Chem 1985; 31: 2018–9. 10.1093/clinchem/31.12.2018Search in Google Scholar

7 Doumas BT, Peters T Jr. Comments on a new biuret reagent. Clin Chem 1977; 23: 1184–6. 10.1093/clinchem/23.6.1184Search in Google Scholar

8 Poge AW, Kirazov L, Kohler H. [Effect of dextrans, amino acids, lipids, bilirubin, hemoglobin, penicillin, heparin and EDTA on the determination of total protein by biuret reaction]. Z Med Lab Diagn 1982; 23: 43–8. Search in Google Scholar

9 Doumas BT, Bayse DD, Carter RJ, Peters T Jr, Schaffer RA. Candidate reference method for determination of total protein in serum. I. Development and validation. Clin Chem 1981; 27: 1642–50. 10.1093/clinchem/27.10.1642Search in Google Scholar

10 Skrede S, Ro JS, Mjolnerod O. Effects of dextrans on the plasma protein changes during the postoperative period. Clin Chim Acta 1973; 48: 143–52. 10.1016/0009-8981(73)90359-8Search in Google Scholar

11 Gofferje H, Rupprecht M. Total blood protein determination by means of the biuret method in the presence of synthetic colloids. Med Welt 1979; 30: 1647–53. Search in Google Scholar

12 Gregor A, Kostrzewska E, Godorowska W. Determination of serum proteins in the presence of dextran by means of the biuret reaction. Infusionsther Klin Ernähr 1977; 4: 48–50. 10.1159/000219790Search in Google Scholar

13 Flack CP, Woollen JW. Prevention of interference by dextran with biuret-type assay of serum proteins. Clin Chem 1984; 30: 559–61. 10.1093/clinchem/30.4.559Search in Google Scholar

14 Magid E, Rønsbo I. Comments on a new biuret reagent. Clin Chem 1977; 23: 1185. Search in Google Scholar

15 Tukey JW. Exploratory data analysis. Reading, MA: Addison-Wesley, 1977:506. Search in Google Scholar

16 Norkus E, Vaiciuniené J, Vuorinen T, Heikkilä M. Interaction of Cu(II) with dextran in alkaline solutions. Carbohyd Polym 2002; 50: 159–64. 10.1016/S0144-8617(02)00056-5Search in Google Scholar

17 Young DS. Effects of drugs on clinical laboratory tests, vol II. Listing by drug. Washington: AACC Press, 2000:3–274. Search in Google Scholar

18 Vercammen M, Goedhuys W, Boeyckens A, De Roy R, Sennesael J, Sevens C, et al. Iron and iron-binding capacity in serum of patients receiving iron-dextran: Kodak Ektachem methodologies, spectrophotometry, and atomic absorption spectrometry compared. Clin Chem 1990; 36: 1812–5. 10.1093/clinchem/36.10.1812Search in Google Scholar

19 Nazar SM, Schmidt H. Beseitigung des Störeinflusses von Dextran auf die Bestimmung von Serumprotein mit der Biuretreaktion durch Dextranase. Z Klin Chem 1972; 10: 548–51. Search in Google Scholar

Received: 2004-7-20
Accepted: 2004-10-22
Published Online: 2011-9-21
Published in Print: 2005-1-1

©2005 by Walter de Gruyter Berlin New York

Articles in the same Issue

  1. Pathophysiology and diagnostic value of urinary trypsin inhibitors
  2. Apolipoprotein H (apoH)-dependent autoantibodies and apoH protein polymorphism in selected patients showing lupus anticoagulant activity
  3. Differential production of immunoglobulin classes and subclasses by mucosal-type human B-lymphocytes exposed in vitro to CpG oligodeoxynucleotides
  4. The predictive power of serum κ/λ ratios for discrimination between monoclonal gammopathy of undetermined significance and multiple myeloma
  5. The soluble transferrin receptor (sTfR)-ferritin index is a potential predictor of celiac disease in children with refractory iron deficiency anemia
  6. Monitoring of fibrinolysis parameters during myocardial revascularization according to type of procedure
  7. Serum amyloid A protein levels as a possible aid in the diagnosis of acute appendicitis in children
  8. External failures as descriptor of quality in clinical microbiology laboratory services
  9. The effects of extracorporeal cardiopulmonary circulation on serum total and free prostate specific antigen levels
  10. Quantitative automated human chorionic gonadotropin measurement in urine using the Modular Analytics E170 module (Roche)
  11. Interference of dextran in biuret-type assays of serum proteins
  12. Evaluation of immunoassays for the measurement of insulin-like growth factor-I and procollagen type III peptide, indirect biomarkers of recombinant human growth hormone misuse in sport
  13. Evaluation of a new automated electrochemiluminescent sex hormone-binding globulin (SHBG) immunoassay
  14. The late Greco-Roman and Byzantine contribution towards the evolution of laboratory examinations of bodily excrement. Part 2: sputum, vomit, blood, sweat, autopsies
  15. Faecal elastase 1 measurement in chronic pancreatitis
  16. On the origin of BÄK requirements for imprecision
  17. Reference intervals for serum thyrotropin (TSH) and free thyroxine (FT4) in adults using the Access ® Immunoassay System
  18. Erythrocyte glutathione and serum total glutathione-S-transferase in epilepsy
  19. CCLM - Guidelines for Authors
Downloaded on 30.12.2025 from https://www.degruyterbrill.com/document/doi/10.1515/CCLM.2005.011/pdf
Scroll to top button