Acid ribonuclease and alkaline ribonuclease isoenzymes in plasma of patients with decreased glomerular filtration rate
-
Abstract
Removal of low molecular weight proteins from plasma by kidneys depends on glomerular filtration rate (GFR), protein-glomerular membrane electric charge, steric interactions and a number of functionally active nephrons present in the kidneys. There is a well documented relationship between the concentration of low molecular weight proteins in plasma and GFR value in patients with impaired renal function. Accumulation of low molecular weight proteins in plasma along with a decrease in GFR value may in the long run enhance formation of protein tissue deposits known as various forms of amyloidosis. In this paper we present studies on plasma concentrations of acid leukocyte-type ribonuclease (RNase) and alkaline pancreatic-type RNase and GFR value in 54 patients with renal failure. RNase isoenzymes' activities were assayed by measuring their enzyme activities manifested as ability to decompose yeast RNA and assay of digestion products' concentration by spectrophotometry. The studies show that decreasing filtration rate produces an increase in serum activities of both acid and alkaline RNases, which is proportional to the logarithm of GFR value. However, the increase rate vs. GFR value is by four times higher for acid RNase then for alkaline RNase. Acid RNase in human plasma is mostly of leukocytic origin and differs from pancreatic-type alkaline RNase, which is of pancreatic origin. The obtained results may suggest that leukocyte originating proteins essentially contribute to low molecular weight protein accumulation in plasma of patients with chronic renal insufficiency.
Copyright (c) 2004 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Phage-displayed recombinant single-chain antibody fragments with high affinity for cholesteryl ester transfer protein (CETP): cDNA cloning, characterization and CETP quantification
- Stem cell factor and macrophage-colony stimulating factor in patients with pancreatic cancer
- Modulation of plasma fibrinogen levels in acute-phase response after hepatectomy
- Anti-nucleosome, anti-chromatin, anti-dsDNA and anti-histone antibody reactivity in systemic lupus erythematosus
- Expression of activation molecules in neutrophils, monocytes and lymphocytes from patients with unstable angina treated with stent implantation
- Acid ribonuclease and alkaline ribonuclease isoenzymes in plasma of patients with decreased glomerular filtration rate
- Is calcium oxalate nucleation in postprandial urine of males with idiopathic recurrent calcium urolithiasis related to calcium phosphate nucleation and the intensity of stone formation? Studies allowing insight into a possible role of urinary free citrate and protein
- Significance of consecutive international normalized ratio (INR) outcomes using statistical control rules in long-term anticoagulated patients. Optimization of laboratory monitoring and interpretation of borderline measurements
- Clinical trials and p-values, beware of the extremes
- Age dependence of serum β-N-acetylhexosaminidase (NAG) activity
- Elevated plasma total homocysteine in centenarians
- New methodology of influential point detection in regression model building for the prediction of metabolic clearance rate of glucose
- Intra-laboratory analytical variability of biochemical markers of fibrosis (Fibrotest) and activity (Actitest) and reference ranges in healthy blood donors
- The glutathione S-transferase polymorphisms in a control population and in Alzheimer's disease patients
- Near-patient testing for infection using urinalysis and immuno-chromatography strips
- Long-distance mountain biking does not disturb the measurement of total, free or complexed prostate-specific antigen in healthy men
- Development of a new colorimetric method for protein S activity measurement
- Re.: Correction to the IFCC primary reference method for the measurement of catalytic activity concentration of enzymes at 37C- part 2: reference procedure for the measurement of creatine kinase. Clin Chem Lab Med 2002;40:635-42
- Meetings and Awards
Articles in the same Issue
- Phage-displayed recombinant single-chain antibody fragments with high affinity for cholesteryl ester transfer protein (CETP): cDNA cloning, characterization and CETP quantification
- Stem cell factor and macrophage-colony stimulating factor in patients with pancreatic cancer
- Modulation of plasma fibrinogen levels in acute-phase response after hepatectomy
- Anti-nucleosome, anti-chromatin, anti-dsDNA and anti-histone antibody reactivity in systemic lupus erythematosus
- Expression of activation molecules in neutrophils, monocytes and lymphocytes from patients with unstable angina treated with stent implantation
- Acid ribonuclease and alkaline ribonuclease isoenzymes in plasma of patients with decreased glomerular filtration rate
- Is calcium oxalate nucleation in postprandial urine of males with idiopathic recurrent calcium urolithiasis related to calcium phosphate nucleation and the intensity of stone formation? Studies allowing insight into a possible role of urinary free citrate and protein
- Significance of consecutive international normalized ratio (INR) outcomes using statistical control rules in long-term anticoagulated patients. Optimization of laboratory monitoring and interpretation of borderline measurements
- Clinical trials and p-values, beware of the extremes
- Age dependence of serum β-N-acetylhexosaminidase (NAG) activity
- Elevated plasma total homocysteine in centenarians
- New methodology of influential point detection in regression model building for the prediction of metabolic clearance rate of glucose
- Intra-laboratory analytical variability of biochemical markers of fibrosis (Fibrotest) and activity (Actitest) and reference ranges in healthy blood donors
- The glutathione S-transferase polymorphisms in a control population and in Alzheimer's disease patients
- Near-patient testing for infection using urinalysis and immuno-chromatography strips
- Long-distance mountain biking does not disturb the measurement of total, free or complexed prostate-specific antigen in healthy men
- Development of a new colorimetric method for protein S activity measurement
- Re.: Correction to the IFCC primary reference method for the measurement of catalytic activity concentration of enzymes at 37C- part 2: reference procedure for the measurement of creatine kinase. Clin Chem Lab Med 2002;40:635-42
- Meetings and Awards
