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Matrix effects in clinical immunoassays and the effect of preheating and cooling analytical samples

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Published/Copyright: June 1, 2005
Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 42 Issue 1

Abstract

Immunological reactions are influenced by various factors including antigens, antibodies and other variables. We focused on two items: i) matrix effects, especially of detergents and ii) temperature effects: preheating sera, especially effects on rheumatoid factor (RF) measurement and false-positive reactions in ELISAs, and cold storage of sera, especially effects on complement. Among various additives, detergents affected the agglutination reaction for fecal hemoglobin and hepatitis B surface (HBs) antigen. Some of the detergents examined abolished these antigenicities, however, polyethylenglycols enhanced the reactions. Heat-inactivation of sera at 56°C for 30 min was employed in serological testing. However, in RF measurement, 10 min of preheating was sufficient to abolish C1q (subcomponent of C1), which could participate in the agglutination reaction. In ELISA for antibodies, false-positive reactions were caused by preheating sera. By the analyses of assays for antibodies to hepatitis C virus (HCV) and cardiolipin, it was found that they were induced by immunoglobulin G (IgG) modified by preheating. Cold storage induced activation of complement (cold activation) in anti-HCV antibody positive sera. CH50 titers in the sera were lowered by one cycle of freezing at −20°C and thawing, and the decrease was affected by the containers.

Published Online: 2005-6-1
Published in Print: 2004-2-16

Copyright © 2004 by Walter de Gruyter GmbH & Co. KG

Articles in the same Issue

  1. International collaboration in clinical chemistry and laboratory medicine: the Human Proteome Organization (HUPO) Plasma Proteome Project
  2. Current concepts in standardization of cardiac marker immunoassays
  3. Collagen, ageing and nutrition
  4. Evaluation of a new efficient procedure for single-nucleotide polymorphism genotyping: tetra-primer amplification refractory mutation system-polymerase chain reaction
  5. High prevalence of non-HFE gene-associated haemochromatosis in patients from southern Italy
  6. Limited usefulness of the PFA-100™ for the monitoring of ADP receptor antagonists–in vitro experience
  7. Isolation of plasma small-dense low-density lipoprotein using a simple air-driven ultracentrifuge and quantification using immunassay of apolipoprotein B
  8. Analytical performance and diagnostic accuracy of a fully-automated electrochemiluminescent assay for the N-terminal fragment of the pro-peptide of brain natriuretic peptide in patients with cardiomyopathy: comparison with immunoradiometric assay methods for brain natriuretic peptide and atrial natriuretic peptide
  9. Calcium oxalate crystallization in untreated urine, centrifuged and filtered urine and ultrafiltered urine
  10. Matrix effects in clinical immunoassays and the effect of preheating and cooling analytical samples
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  12. Determination of lipase catalytic activity in two reference materials: BCR 693 and BCR 694 by titrimetry at constant pH
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  18. Evaluation of the blood gas analyzer GEM(r) PREMIER(tm) 3000
  19. Lactate dehydrogenase (LD) extra isoenzyme electrophoretic band between LD1 and LD2 caused by a complex with α1-lipoprotein. A case report
  20. Relationship between cryoglobulins and hepatitis C virus (HCV) core antigen or antibody titers
  21. Spurious hyperphosphatemia due to sample contamination with heparinized saline from an indwelling catheter
  22. Biological variation of plasma chromogranin A
  23. Verification, validation and evaluation of analytical procedures in laboratory medicine
  24. DACH-LIGA Homocystein (German, Austrian and Swiss Homocysteine Society): Consensus Paper on the Rational Clinical Use of Homocysteine, Folic Acid and B-Vitamins in Cardiovascular and Thrombotic Diseases: Guidelines and Recommendations
  25. Meetings and Awards
https://doi.org/10.1515/CCLM.2004.010

Articles in the same Issue

  1. International collaboration in clinical chemistry and laboratory medicine: the Human Proteome Organization (HUPO) Plasma Proteome Project
  2. Current concepts in standardization of cardiac marker immunoassays
  3. Collagen, ageing and nutrition
  4. Evaluation of a new efficient procedure for single-nucleotide polymorphism genotyping: tetra-primer amplification refractory mutation system-polymerase chain reaction
  5. High prevalence of non-HFE gene-associated haemochromatosis in patients from southern Italy
  6. Limited usefulness of the PFA-100™ for the monitoring of ADP receptor antagonists–in vitro experience
  7. Isolation of plasma small-dense low-density lipoprotein using a simple air-driven ultracentrifuge and quantification using immunassay of apolipoprotein B
  8. Analytical performance and diagnostic accuracy of a fully-automated electrochemiluminescent assay for the N-terminal fragment of the pro-peptide of brain natriuretic peptide in patients with cardiomyopathy: comparison with immunoradiometric assay methods for brain natriuretic peptide and atrial natriuretic peptide
  9. Calcium oxalate crystallization in untreated urine, centrifuged and filtered urine and ultrafiltered urine
  10. Matrix effects in clinical immunoassays and the effect of preheating and cooling analytical samples
  11. Blood alcohol testing: comparison of the performance obtained with the different methods used in the Belgian external quality assessment schemes
  12. Determination of lipase catalytic activity in two reference materials: BCR 693 and BCR 694 by titrimetry at constant pH
  13. Evaluation of a new generation of plastic evacuated blood-collection tubes in clinical chemistry, therapeutic drug monitoring, hormone and trace metal analysis
  14. Association between the CYP2C9 polymorphism and the drug metabolism phenotype
  15. Analytical goals for coagulation tests based on biological variation
  16. In vivo total antioxidant capacity: comparison of two different analytical methods
  17. Performance evaluation of automated assays for β-CrossLaps, N-MID-Osteocalcin and intact parathyroidhormone (BIOROSE Multicenter Study)
  18. Evaluation of the blood gas analyzer GEM(r) PREMIER(tm) 3000
  19. Lactate dehydrogenase (LD) extra isoenzyme electrophoretic band between LD1 and LD2 caused by a complex with α1-lipoprotein. A case report
  20. Relationship between cryoglobulins and hepatitis C virus (HCV) core antigen or antibody titers
  21. Spurious hyperphosphatemia due to sample contamination with heparinized saline from an indwelling catheter
  22. Biological variation of plasma chromogranin A
  23. Verification, validation and evaluation of analytical procedures in laboratory medicine
  24. DACH-LIGA Homocystein (German, Austrian and Swiss Homocysteine Society): Consensus Paper on the Rational Clinical Use of Homocysteine, Folic Acid and B-Vitamins in Cardiovascular and Thrombotic Diseases: Guidelines and Recommendations
  25. Meetings and Awards
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