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Genotyping of Alcohol Dehydrogenase Type 2 and 3 Using a Two-Buffer Polyacrylamide Gel Electrophoresis System

  • Robea Ballo , Dong-Ping Li and M. Iqbal Parker
Published/Copyright: June 1, 2005
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Volume 41 Issue 3

Abstract

Genetic polymorphisms in the alcohol dehydrogenase genes, ADH2 and ADH3, have been shown to affect individual susceptibility to diseases such as alcoholism and oesophageal cancer. Although several PCR-based methods for genotyping these enzymes have been previously developed, the two-buffer polyacrylamide gel electrophoresis system has the ability to rapidly resolve all classes of point mutations within 2–3 hours instead of the conventional overnight separation. The success of this technique is partly attributable to a discontinuous two-phase buffer system and horizontal flatbed gel electrophoresis rather than conventional vertical gels. Using a modification of this system, we were able to detect all of the known polymorphisms within ADH2 exons 3 and 9 and ADH3 exon 8, as well as a rare variant within ADH2 exon 9. This method is rapid, cost-effective, and is ideal for large scale screening programmes.

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Published Online: 2005-06-01
Published in Print: 2003-03-24

Copyright © 2003 by Walter de Gruyter GmbH & Co. KG

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https://doi.org/10.1515/CCLM.2003.047

Articles in the same Issue

  1. 3rd Conference on Hyperhomocysteinemia Saarbrücken, Germany, 11–12 April 2003, Saarland University
  2. Platelet Receptors and Their Role in Diseases
  3. Real-Time RT-PCR for the Measurement of Prostate-Specific Antigen mRNA Expression in Benign Hyperplasia and Adenocarcinoma of Prostate
  4. Detection of a Novel Exon 4 Low-Density Lipoprotein Receptor Gene Deletion in a Swiss Family with Severe Familial Hypercholesterolemia
  5. Absence of Known Familial Hemiplegic Migraine (FHM) Mutations in the CACNA1A Gene in Patients with common Migraine: Implications for Genetic Testing
  6. Association between 5,10-Methylenetetrahydrofolate Reductase C677T and A1298C Polymorphisms and Conotruncal Heart Defects
  7. Rapid and Reliable Genotyping of the C181T Polymorphism in the Bradykinin B2 Receptor Gene
  8. Steroids, Sex Hormone-Binding Globulin, Homocysteine, Selected Hormones and Markers of Lipid and Carbohydrate Metabolism in Patients with Severe Hypothyroidism and Their Changes Following Thyroid Hormone Supplementation
  9. Anaerobic Bacteremia: The Yield of Positive Anaerobic Blood Cultures: Patient Characteristics and Potential Risk Factors
  10. Genotyping of Alcohol Dehydrogenase Type 2 and 3 Using a Two-Buffer Polyacrylamide Gel Electrophoresis System
  11. Effect of Partial Proteolysis on the Activation Energy of β-N-Acetylhexosaminidase Precursor and Mature Forms
  12. Angiogenin and Gestational Trophoblastic Tumors, a Promising Prognostic Marker
  13. Carbohydrate 19-9 Antigen Is Not a Marker of Liver Disease in Patients with Cystic Fibrosis
  14. Assay Using Succinyldithiocholine as Substrate: The Method of Choice for the Measurement of Cholinesterase Catalytic Activity in Serum to Diagnose Succinyldicholine Sensitivity
  15. National External Quality Assessment Scheme for Lymphocyte Immunophenotyping in Belgium
  16. Quality Assurance for Cerebrospinal Fluid Protein Analysis: International Consensus by an Internet-Based Group Discussion
  17. Guidelines for the Analysis of Bence Jones Protein
  18. Discordance Rate, a New Concept for Combining Diagnostic Decisions with Analytical Performance Characteristics. 1. Application in Method or Sample System Comparisons and in Defining Decision Limits
  19. Urinary Homogentisic Acid in Alkaptonuric and Healthy Children
  20. The Influence of Hemolysis, Turbidity and Icterus on the Measurements of CK-MB, Troponin I and Myoglobin
  21. Evaluation of Cobas Integra® 800 under Simulated Routine Conditions in Six Laboratories
  22. Guidelines for the Laboratory Investigation of Inherited Thrombophilias. Recommendations for the First Level Clinical Laboratories
  23. Detection of PvuII Polymorphism within Intron 1 of ESR1 Gene by Real-Time PCR
  24. Meetings and Awards
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