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Assessment of Intestinal Permeability: Enzymatic Determination of Urinary Mannitol, Raffinose, Sucrose and Lactose on Hitachi Analyzer
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Jan Hessels
Veröffentlicht/Copyright:
1. Juni 2005
Abstract
The sugar absorption test is the usual test for measurement of intestinal permeability. After intestinal absorption of probe sugars the subsequently excreted sugars are measured in urine. We have developed four enzymatic methods for the measurement of the urinary concentration of the probe sugars mannitol, raffinose, lactose and sucrose. Mannitol, lactose and sucrose are directly measured on Hitachi 917 using mannitol dehydrogenase, β-galactosidase and invertase, respectively, as enzyme reagents. Raffinose measurement needs a three hours preincubation with α-galactosidase, after which the liberated sucrose is measured. The analytical performances such as within- and between-run precision, linearity, lowest detection limit, interference of other sugars and comparison with a gas chromatographic method are described for the four methods. These methods are accurate an can easily be performed in any clinical laboratory.
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Published Online: 2005-06-01
Published in Print: 2003-01-27
Copyright © 2003 by Walter de Gruyter GmbH & Co. KG
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Artikel in diesem Heft
- Evidence-Based Laboratory Medicine: Addressing Bias, Generalisability and Applicability in Studies on Diagnostic Accuracy. The STARD Initiative
- Semen Polymorphonuclear Neutrophil Leukocyte Elastase as a Diagnostic and Prognostic Marker of Genital Tract Inflammation – A Review
- Total Oxyradical Scavenging Capacity toward Different Reactive Oxygen Species in Seminal Plasma and Sperm Cells
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- 3-Nitrotyrosine in Atherosclerotic Blood Vessels
- Molecular Diagnosis of Cystic Fibrosis: Comparison of Four Analytical Procedures
- Assessment of Intestinal Permeability: Enzymatic Determination of Urinary Mannitol, Raffinose, Sucrose and Lactose on Hitachi Analyzer
- Effects of Calcium Carbonate, Magnesium Oxide and Sodium Citrate Bicarbonate Health Supplements on the Urinary Risk Factors for Kidney Stone Formation
- Glycerol Metabolism and the Determination of Triglycerides –Clinical, Biochemical and Molecular Findings in Six Subjects
- Plasma Lipid Composition and LDL Oxidation
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- Towards Complete and Accurate Reporting of Studies of Diagnostic Accuracy: The STARD Initiative
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