Evaluation of an Automated Spectrophotometric Assay for Reactive Oxygen Metabolites in Serum
-
Luigia Iamele
Abstract
The in vivo assessment of free radicals concentration is hampered by their instability and extremely short half-life. The Diacron Reactive Oxygen Metabolites (D-ROM) test is a recently introduced method to evaluate the peroxidation of organic compounds. Since the manual performance of the test provides excessive analytical imprecision, the aim of this study was to evaluate the automation of this test. Within- and between-run imprecision and interference were assessed according to the guidelines proposed by the NCCLS. The reactive oxygen metabolites' (ROM) stability was evaluated in different physical conditions. For withinrun and between-run imprecision the coefficients of variation were consistently lower than 5%. The maximum allowable concentration was 28.2 mmol/l, 0.068 mmol/l and 171 mmol/l for triglycerides, haemoglobin and bilirubin, respectively. Serum storage at −20°C provided adequate ROM stability for up to 3 months, whereas storage at 4°C yielded non-reproducible results. In conclusion, our data provide evidence that the D-ROM assay has both an acceptable stability and an adequate imprecision. The automated assay may be regarded as a fast and reproducible method for the quantitative evaluation of oxidative stress. Since it is easily performed, the method is suitable for routine in clinical laboratories and may provide an accurate estimation of oxidative stress in vivo.
Copyright © 2002 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Mechanisms of Hyperammonemia
- Neopterin – an Early Marker of Surgical Stress and Hypoxic Reperfusion Damage during Liver Surgery
- Isolation of Fetal Nucleated Red Blood Cells from Maternal Blood in Normal and Aneuploid Pregnancies
- Evaluation of an Automated Spectrophotometric Assay for Reactive Oxygen Metabolites in Serum
- The Paraoxonase-1 Codon192 Polymorphism Is Associated with Fasting Total Cholesterol and LDL-Cholesterol Concentrations only in Postmenopausal Women.The REGICOR Study
- Serum Oxidant/Antioxidant Status in Patients with Systemic Lupus Erythematosus
- Frequencies of C28Y and H63D Mutations and Transferrin Saturation Indices in the Korean Population
- Validation of the Phenylalanine/Tyrosine Ratio Determined by Tandem Mass Spectrometry: Sensitive Newborn Screening for Phenylketonuria
- Multicenter Study of the LCx® HIV RNA Quantitative Assay – a New Competitive Reverse Transcriptase-PCR which Targets pol Genomic Region of HIV-1 for the Measurement of Type B, Non-Type B and Group O HIV-1 RNA
- The Interference of Insulin Antibodies in Insulin Immunometric Assays
- A Comparison of Different Sample Matrices for Evaluating Functional Sensitivity, Imprecision and Dilution Linearity of the Abbott ARCHITECT® i2000® TSH Assay
- Determination of the Length of Sedimentation Reaction (Erythrocyte Sedimentation Rate) in Non-Anticoagulated Blood with the Microtest 1
- IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37°C. Part 4. Reference Procedure for the Measurement of Catalytic Concentration of Alanine Aminotransferase
- IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37°C. Part 5. Reference Procedure for the Measurement of Catalytic Concentration of Aspartate Aminotransferase
- IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37C. Part 6. Reference Procedure for the Measurement of Catalytic Concentration of γ-Glutamyltransferase
- IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37°C. Part 7. Certification of Four Reference Materials for the Determination of Enzymatic Activity of γ-Glutamyltransferase, Lactate Dehydrogenase, Alanine Aminotransferase and Creatine Kinase according to IFCC Reference Procedures at 37°C
- Clinical Biochemistry: Techniques and Instrumentation – a Practical Course. Edited by John S. Varcoe
- Meetings and Awards
Articles in the same Issue
- Mechanisms of Hyperammonemia
- Neopterin – an Early Marker of Surgical Stress and Hypoxic Reperfusion Damage during Liver Surgery
- Isolation of Fetal Nucleated Red Blood Cells from Maternal Blood in Normal and Aneuploid Pregnancies
- Evaluation of an Automated Spectrophotometric Assay for Reactive Oxygen Metabolites in Serum
- The Paraoxonase-1 Codon192 Polymorphism Is Associated with Fasting Total Cholesterol and LDL-Cholesterol Concentrations only in Postmenopausal Women.The REGICOR Study
- Serum Oxidant/Antioxidant Status in Patients with Systemic Lupus Erythematosus
- Frequencies of C28Y and H63D Mutations and Transferrin Saturation Indices in the Korean Population
- Validation of the Phenylalanine/Tyrosine Ratio Determined by Tandem Mass Spectrometry: Sensitive Newborn Screening for Phenylketonuria
- Multicenter Study of the LCx® HIV RNA Quantitative Assay – a New Competitive Reverse Transcriptase-PCR which Targets pol Genomic Region of HIV-1 for the Measurement of Type B, Non-Type B and Group O HIV-1 RNA
- The Interference of Insulin Antibodies in Insulin Immunometric Assays
- A Comparison of Different Sample Matrices for Evaluating Functional Sensitivity, Imprecision and Dilution Linearity of the Abbott ARCHITECT® i2000® TSH Assay
- Determination of the Length of Sedimentation Reaction (Erythrocyte Sedimentation Rate) in Non-Anticoagulated Blood with the Microtest 1
- IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37°C. Part 4. Reference Procedure for the Measurement of Catalytic Concentration of Alanine Aminotransferase
- IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37°C. Part 5. Reference Procedure for the Measurement of Catalytic Concentration of Aspartate Aminotransferase
- IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37C. Part 6. Reference Procedure for the Measurement of Catalytic Concentration of γ-Glutamyltransferase
- IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37°C. Part 7. Certification of Four Reference Materials for the Determination of Enzymatic Activity of γ-Glutamyltransferase, Lactate Dehydrogenase, Alanine Aminotransferase and Creatine Kinase according to IFCC Reference Procedures at 37°C
- Clinical Biochemistry: Techniques and Instrumentation – a Practical Course. Edited by John S. Varcoe
- Meetings and Awards
