Plasma Total Antioxidant Capacity in Hemodialyzed Patients and Its Relationships to other Biomarkers of Oxidative Stress and Lipid Peroxidation
-
GianMario Gerardi
Abstract
Patients undergoing long-term hemodialysis (HD) exhibit increased levels of oxidative stress, likely contributing to the increased rate of cardiovascular disease. The present study represents a critical evaluation of some of the most widely used oxidative indicators, as applied to the monitoring of hemodialysis-associated oxidative stress.
Total plasma antioxidant capacity was determined by two independent procedures, the total antioxidant status (TAS) and the ferric reducing ability of plasma (FRAP) methods. Plasma lipid peroxidation was assessed by determining the peroxidation products malonaldehyde and 4-hydroxynonenal (MDA-4HNE) as well as lipid hydroperoxides (“Fox-2” and “d-ROMs” methods). Total plasma thiols and plasma b-tocopherol were also determined.
MDA-4HNE levels were higher in HD patients and decreased following HD, possibly due to passive diffusion across dialysis filters. d-ROMs were also higher in HD patients but exhibited a further increase following the dialysis procedure. Serum α-tocopherol did not show any significant differences. Plasma thiols were lower in HD patients and were restored following HD. Plasma total antioxidant capacity determined with either method was unexpectedly higher in HD patients compared to controls, and decreased following HD.
These data indicate that, of the biomarkers studied, d-ROMs level is the one more accurately reflecting the oxidative alterations taking place in HD patients, while determination of MDA-4HNE fails to detect oxidation occurring during the HD sessions. In addition, our findings point out that the determination of total antioxidant capacity in HD patients is severely affected by the concomitant fluctuations in plasma urate levels and therefore needs careful interpretation.
Copyright © 2002 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Recommendations for the Routine Use of Pancreatic Amylase Measurement instead of Total Amylase for the Diagnosis and Monitoring of Pancreatic Pathology
- Bovine Spongiform Encephalopathy: Are the Cows Mad or Full of Carbohydrates?
- Plasma Total Antioxidant Capacity in Hemodialyzed Patients and Its Relationships to other Biomarkers of Oxidative Stress and Lipid Peroxidation
- Effects of Propofol and Taurine on Intracellular Free Amino Acid Profiles and Immune Function Markers in Neutrophils In Vitro
- Genotyping of Hemochromatosis-Associated Mutations in the HFE Gene by PCR-RFLP and a Novel Revers Hybridization Method
- Comparison of Different CD71 Monoclonal Antibodies for Enrichment of Fetal Cells from Maternal Blood
- The Effects of Marathon Swimming on Serum Leptin and Plasma Neuropeptide Y Levels
- Renal Function Exerts only a Minor Influence on High Plasma Homocysteine Concentrations in Patients with Acute Coronary Syndromes
- Changes of Biochemical Bone Markers during the Menopausal Transition
- Blood Sampling for Biochemical Analysis from Central Venous Catheters: Minimizing the Volume of Discarded Blood
- Experience with External Quality Assessment of Drugs of Abuse Testing in the Lombardy Region in Italy
- Reference Intervals for Thyroid Hormones on the Architect Analyser
- Intermethod Variation in Serum Carcinoembryonic Antigen (CEA) Measurement. Fresh Serum Pools and Control Materials Compared
- Turn-Around Time for Chemical and Endocrinology Analyzers Studied Using Simulation
- A Two-Center Evaluation of the Blood Gas Immediate Response Mobile Analyzer (IRMA)
- Painting, Poetry and Optics: Johannes Vermeer
- The Practice of Clinical Chemistry in the European Union
- Meetings and Awards
Articles in the same Issue
- Recommendations for the Routine Use of Pancreatic Amylase Measurement instead of Total Amylase for the Diagnosis and Monitoring of Pancreatic Pathology
- Bovine Spongiform Encephalopathy: Are the Cows Mad or Full of Carbohydrates?
- Plasma Total Antioxidant Capacity in Hemodialyzed Patients and Its Relationships to other Biomarkers of Oxidative Stress and Lipid Peroxidation
- Effects of Propofol and Taurine on Intracellular Free Amino Acid Profiles and Immune Function Markers in Neutrophils In Vitro
- Genotyping of Hemochromatosis-Associated Mutations in the HFE Gene by PCR-RFLP and a Novel Revers Hybridization Method
- Comparison of Different CD71 Monoclonal Antibodies for Enrichment of Fetal Cells from Maternal Blood
- The Effects of Marathon Swimming on Serum Leptin and Plasma Neuropeptide Y Levels
- Renal Function Exerts only a Minor Influence on High Plasma Homocysteine Concentrations in Patients with Acute Coronary Syndromes
- Changes of Biochemical Bone Markers during the Menopausal Transition
- Blood Sampling for Biochemical Analysis from Central Venous Catheters: Minimizing the Volume of Discarded Blood
- Experience with External Quality Assessment of Drugs of Abuse Testing in the Lombardy Region in Italy
- Reference Intervals for Thyroid Hormones on the Architect Analyser
- Intermethod Variation in Serum Carcinoembryonic Antigen (CEA) Measurement. Fresh Serum Pools and Control Materials Compared
- Turn-Around Time for Chemical and Endocrinology Analyzers Studied Using Simulation
- A Two-Center Evaluation of the Blood Gas Immediate Response Mobile Analyzer (IRMA)
- Painting, Poetry and Optics: Johannes Vermeer
- The Practice of Clinical Chemistry in the European Union
- Meetings and Awards
