Soluble Transferrin Receptor (sTfR): Biological Variations and Reference Limits
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Ghiath Raya
Abstract
The aim of this study was to establish soluble serum transferrin receptor (sTfR) reference limits. sTfR was measured in 885 healthy subjects from 3 to 91 years old (433 men, 409 women), without hematological abnormalities, using an immunonephelometric assay.
The sTfR median concentrations in our population decreased gradually from the group aged 3–10 years to the group aged 21–40 years, then there were no changes in the older groups except for the females >60 years of age.
The interindividual variability ranged from 12.6% to 30.3% among different age groups, and the analytical variability was 5%.
Biological factors and other factors associated with sTfR concentration variation were examined and accounted for 35% of the sTfR variability in men aged 20 years or less, and 18% in those older than 20 years. Also, they accounted for 45% of the variability in women aged 20 years or less and 14% in those older than 20 years.
The main factors statistically associated with sTfR concentration in males were ferritin, orosomucoid, hemoglobin, and tobacco in all age groups and only mean corpuscular volume (MCV) in males less than 20 years old. In the females the main factors were age, orosomucoid, and hemoglobin in all age groups, MCV and tobacco in females less than 20 years old, and ferritin and physical activity in females more than 20 years old.
These factors were used to define the exclusion and partition criteria for obtaining the reference samples. Medians for reference values were: 1.60 mg/l in the 3–10-year old group (males and females); 1.42 mg/l in males between 11 and 20 years of age, and 1.33 mg/l in females of the same age. In the other age groups, the median of the reference values was 1.16 mg/l, except in females over 60 years old, for whom it was 1.26 mg/l.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- The Present State of Protein Analysis and Interpretation
- In Memoriam Carl-Bertil Laurell
- From Paper Electrophoresis to Computer-supported Interpretation of Capillary Electrophoresis – Clinical Plasma Protein Analysis in Malmö, Sweden
- The Pavia Approach to Clinical Protein Analysis
- How the Foundation for Blood Research (FBR) Has Managed Serum Protein Testing for New England Clinicians
- Evidence-Based Laboratory Interpretation System Built on a Large Collection of Case Records with Well-Defined Diagnoses
- A Knowledge-Based System to Aid with the Clinical Interpretation of Complex Serum Protein Data
- Markers of the Acute Phase Response in Cardiovascular Disease: An Update
- Protein Aggregation
- Protein Standardization I: Protein Purification. Procedure for the Purification of Human Prealbumin, Orosomucoid and Transferrin as Primary Protein Preparations
- Protein Standardization II: Dry Mass Determination. Procedure for the Determination of the Dry Mass of a Pure Protein Preparation
- Protein Standardization III: Method Optimization. Basic Principles for Quantitative Determination of Human Serum Proteins on Automated Instruments Based on Turbidimetry or Nephelometry
- Protein Standardization IV: Value Transfer. Procedure for the Assignment of Serum Protein Values from a Reference Preparation to a Target Material
- Effect of Certified Reference Material 470 (CRM 470) on National Quality Assurance Programs for Serum Proteins in Europe
- Commutability of Serum Protein Values: Persisting Bias among Manufacturers Using Values Assigned from the Certified Reference Material 470 (CRM 470) in the United States
- The Existing Interim Consensus Reference Ranges and the Future Approach
- Using Multiples of the Median to Normalize Serum Protein Measurements
- Strategy for Determining Racial and Environmental Similarities and Differences for Plasma Proteins
- Standardization of Immunoassay for CRM-Related Proteins in Japan: From Evaluating CRM 470 to Setting Reference Intervals
- Soluble Transferrin Receptor (sTfR): Biological Variations and Reference Limits
- High Sensitivity C-Reactive Protein (CRP) Reference Intervals in the Elderly
- High Sensitivity Immunoassays for C-Reactive Protein: Promises and Pitfalls
- Meetings and Awards
Articles in the same Issue
- The Present State of Protein Analysis and Interpretation
- In Memoriam Carl-Bertil Laurell
- From Paper Electrophoresis to Computer-supported Interpretation of Capillary Electrophoresis – Clinical Plasma Protein Analysis in Malmö, Sweden
- The Pavia Approach to Clinical Protein Analysis
- How the Foundation for Blood Research (FBR) Has Managed Serum Protein Testing for New England Clinicians
- Evidence-Based Laboratory Interpretation System Built on a Large Collection of Case Records with Well-Defined Diagnoses
- A Knowledge-Based System to Aid with the Clinical Interpretation of Complex Serum Protein Data
- Markers of the Acute Phase Response in Cardiovascular Disease: An Update
- Protein Aggregation
- Protein Standardization I: Protein Purification. Procedure for the Purification of Human Prealbumin, Orosomucoid and Transferrin as Primary Protein Preparations
- Protein Standardization II: Dry Mass Determination. Procedure for the Determination of the Dry Mass of a Pure Protein Preparation
- Protein Standardization III: Method Optimization. Basic Principles for Quantitative Determination of Human Serum Proteins on Automated Instruments Based on Turbidimetry or Nephelometry
- Protein Standardization IV: Value Transfer. Procedure for the Assignment of Serum Protein Values from a Reference Preparation to a Target Material
- Effect of Certified Reference Material 470 (CRM 470) on National Quality Assurance Programs for Serum Proteins in Europe
- Commutability of Serum Protein Values: Persisting Bias among Manufacturers Using Values Assigned from the Certified Reference Material 470 (CRM 470) in the United States
- The Existing Interim Consensus Reference Ranges and the Future Approach
- Using Multiples of the Median to Normalize Serum Protein Measurements
- Strategy for Determining Racial and Environmental Similarities and Differences for Plasma Proteins
- Standardization of Immunoassay for CRM-Related Proteins in Japan: From Evaluating CRM 470 to Setting Reference Intervals
- Soluble Transferrin Receptor (sTfR): Biological Variations and Reference Limits
- High Sensitivity C-Reactive Protein (CRP) Reference Intervals in the Elderly
- High Sensitivity Immunoassays for C-Reactive Protein: Promises and Pitfalls
- Meetings and Awards
