Hippuric Acid Test Using 13C-Labelling and NMR Spectroscopy
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Kazuki Akira
Abstract
This article describes a 13C-labelling and nuclear magnetic resonance approach for hippuric acid test which is potentially useful for evaluating liver reserve. In this approach, urine samples collected after ingestion of 13C-labelled benzoic acid were directly analysed by 13C nuclear magnetic resonance spectroscopy, and the excreted 13C-labelled hippuric acid formed from the administered benzoic acid was quantitated. The amount of labelled hippuric acid excreted in a specified time can be a useful index of liver reserve. In this study, the feasibility of the nuclear magnetic resonance approach has been investigated in several healthy subjects. This approach is simple and convenient compared with conventional analytical procedures, because no chromatographic separation is required. The approach could give new insights into the liver reserve, because the benzoic acid conversion to hippuric acid intimately relates to the hepatic energy metabolism. This measurement can be conducted at a wide range of dosages without interference from endogenous hippuric acid.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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- Autoantibodies Associated with Rheumatic Diseases
- Simultaneous Detection of Multiple Proteins with an Array-Based Enzyme-Linked Immunosorbent Assay (ELISA) and Enhanced Chemiluminescence (ECL)
- Hippuric Acid Test Using 13C-Labelling and NMR Spectroscopy
- Hippuric Acid as a Modifier of Calcium Oxalate Crystallisation
- Excretion of Sweat and Urine Pyridinoline Crosslinks in Healthy Controls and Subjects with Established Metabolic Bone Disease
- Hyperhomocysteinemia and Changed Plasma Thiol Redox Status in Chronic Obstructive Pulmonary Disease
- Biochemical Evaluation of Oxidative Stress during Exercise in Patients with Coronary Heart Disease
- Prognostic Significance of the Presence of Erythroblasts in Blood after Cardiothoracic Surgery
- Exploratory Biochemical Data Analysis: a Comparison of Two Sample Means and Diagnostic Displays
- Standardization of Laboratory Data and Establishment of Reference Intervals in the Fukuoka Prefecture: A Japanese Perspective
- Calibration, Specificity and Trueness of a Postheparin Plasma Lipoprotein Lipase Assay
- Analytical Performance of a Direct Assay for LDL-Cholesterol
- Genotyping Method for Point Mutation Detection in the Endothelial Nitric Oxide Synthase Exon 7 Using Fluorescent Probes. Clinical Validation in Systemic Sclerosis Patients
- IFCC Reference Measurement Procedure for Substance Concentration Determination of Total Carbon Dioxide in Blood, Plasma or Serum
- Joshua Lederberg (Editor-in-Chief): Encyclopedia of Microbiology, 2nd Edition, Vol 1-4
Articles in the same Issue
- Autoantibodies Associated with Rheumatic Diseases
- Simultaneous Detection of Multiple Proteins with an Array-Based Enzyme-Linked Immunosorbent Assay (ELISA) and Enhanced Chemiluminescence (ECL)
- Hippuric Acid Test Using 13C-Labelling and NMR Spectroscopy
- Hippuric Acid as a Modifier of Calcium Oxalate Crystallisation
- Excretion of Sweat and Urine Pyridinoline Crosslinks in Healthy Controls and Subjects with Established Metabolic Bone Disease
- Hyperhomocysteinemia and Changed Plasma Thiol Redox Status in Chronic Obstructive Pulmonary Disease
- Biochemical Evaluation of Oxidative Stress during Exercise in Patients with Coronary Heart Disease
- Prognostic Significance of the Presence of Erythroblasts in Blood after Cardiothoracic Surgery
- Exploratory Biochemical Data Analysis: a Comparison of Two Sample Means and Diagnostic Displays
- Standardization of Laboratory Data and Establishment of Reference Intervals in the Fukuoka Prefecture: A Japanese Perspective
- Calibration, Specificity and Trueness of a Postheparin Plasma Lipoprotein Lipase Assay
- Analytical Performance of a Direct Assay for LDL-Cholesterol
- Genotyping Method for Point Mutation Detection in the Endothelial Nitric Oxide Synthase Exon 7 Using Fluorescent Probes. Clinical Validation in Systemic Sclerosis Patients
- IFCC Reference Measurement Procedure for Substance Concentration Determination of Total Carbon Dioxide in Blood, Plasma or Serum
- Joshua Lederberg (Editor-in-Chief): Encyclopedia of Microbiology, 2nd Edition, Vol 1-4
