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Determination of Glycerol in Plasma by an Automated Enzymatic Spectrophotometric Procedure
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Rong Li
Published/Copyright:
June 1, 2005
Abstract
The plasma concentration of glycerol, the backbone of triglycerides and the end product of triacylglycerol breakdown, is considered to reflect lipolysis in adipose tissue. We evaluated an automated enzymatic procedure for the measurement of glycerol in plasma. The assay was linear up to 250μmol/l. The detection limit was 8μmol/l. Recovery averaged 94% from spiked plasma and 104% from diluted plasma. An extensive precision study performed according to the guidelines of the National Committee for Clinical Laboratory Standards showed within-run coefficients of variation between 14.8% and 2.6% for concentrations ranging from 28μmol/l to 164 μmol/l. The reference range for fasting healthy adult men was 14–69 μmol/l. Glycerol levels were significantly correlated with free fatty acid levels. This automated enzymatic method is rapid and reliable, and provides greater sensitivity or convenience than previously described procedures.
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Published Online: 2005-06-01
Published in Print: 2001-02-21
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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