Article
Licensed
Unlicensed
Requires Authentication
Simple Gas Chromatography Analysis of Faecal Butyrate: Application to Patients at Risk of Pouchitis
-
Gilles Renom
Published/Copyright:
June 1, 2005
Abstract
In spite of the fact that pouchitis is the most frequently occurring and troublesome complication found in patients treated by ileo-anal anastomosis for ulcerative colitis, no biological marker currently exists to monitor the outcome of the disease. Since it has been noted faecal butyrate is reduced in patients with pouchitis, we developed a simple gas chromatography method to quantify butyrate in faecal water. This test is based on diethyl ether extraction with the use of methacrylic acid as an internal standard. We demonstrated that butyrate was effectively measured when this technique was applied to eleven patients with ileal-pouch anal anastomosis within the first year after the closure of their ileostomy. We also observed a noticeable reduction in the concentration of butyrate in patients who went on to develop a pouchitis.
:
Published Online: 2005-06-01
Published in Print: 2001-02-21
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Editorial
- Polymorphisms in the Apolipoprotein E Gene Regulatory Region in Relation to Coronary Heart Disease and Their Effect on Plasma Apolipoprotein E
- Further Characterization of Serum Amyloid A4 as a Minor Acute Phase Reactant and a Possible Nutritional Marker
- Determination of Human Angiotensin Converting Enzyme (ACE) Gene Polymorphisms in Erectile Dysfunction: Frequency Differences of ACE Gene Polymorphisms according to the Method of Analysis
- Simple Gas Chromatography Analysis of Faecal Butyrate: Application to Patients at Risk of Pouchitis
- Determination of Glycerol in Plasma by an Automated Enzymatic Spectrophotometric Procedure
- Clinical Applications of the 2nd Generation Assay for Anti-TSH Receptor Antibodies in Graves' Disease. Evaluation in Patients with Negative 1st Generation Test
- The Clinical Value of Microtransferrinuria and Microalbuminuria in the Prediction of Pre-eclampsia
- A Non-Icteric Cholecystectomized Patient with Recurrent Attacks of Right Epigastric Pain and Dilated Common Bile Duct – Do Liver Function Tests Predict Bile Duct Stones?
- Serum Lactate Dehydrogenase Isoenzyme 1 and Prediction of Death in Patients with Metastatic Testicular Germ Cell Tumors
- Annual Rhythmic and Non-Rhythmic Biological Variation of Magnesium and Ionized Calcium Concentrations
- Unit-Independent Reporting of Laboratory Test Results
- Analysis of Large and Small Samples of Biochemical and Clinical Data.
- Evaluation of Five Commercial Enzyme Immunoassays for the Detection of Human Cytomegalovirus-Specific IgM Antibodies in the Absence of a Commercially Available Gold Standard
- Alkaline Phosphatase Activity: New Assay for the Reflotron® System. Results of the Evaluation in Eight Clinical Laboratories
- Report on the 4th Estonian Summer School of Laboratory Medicine
- Laboratory-Related Measures of Patient Outcomes: An Introduction. Edited by Michael G. Bissell
- Report from the 50th Anniversary Congress of the Hungarian Society of Clinical Pathology and from the Executive Board Meeting of FESCC in Debrecen (Hungary)
- Report from the 8th Meeting of the Balkan Clinical Laboratory Federation
Articles in the same Issue
- Editorial
- Polymorphisms in the Apolipoprotein E Gene Regulatory Region in Relation to Coronary Heart Disease and Their Effect on Plasma Apolipoprotein E
- Further Characterization of Serum Amyloid A4 as a Minor Acute Phase Reactant and a Possible Nutritional Marker
- Determination of Human Angiotensin Converting Enzyme (ACE) Gene Polymorphisms in Erectile Dysfunction: Frequency Differences of ACE Gene Polymorphisms according to the Method of Analysis
- Simple Gas Chromatography Analysis of Faecal Butyrate: Application to Patients at Risk of Pouchitis
- Determination of Glycerol in Plasma by an Automated Enzymatic Spectrophotometric Procedure
- Clinical Applications of the 2nd Generation Assay for Anti-TSH Receptor Antibodies in Graves' Disease. Evaluation in Patients with Negative 1st Generation Test
- The Clinical Value of Microtransferrinuria and Microalbuminuria in the Prediction of Pre-eclampsia
- A Non-Icteric Cholecystectomized Patient with Recurrent Attacks of Right Epigastric Pain and Dilated Common Bile Duct – Do Liver Function Tests Predict Bile Duct Stones?
- Serum Lactate Dehydrogenase Isoenzyme 1 and Prediction of Death in Patients with Metastatic Testicular Germ Cell Tumors
- Annual Rhythmic and Non-Rhythmic Biological Variation of Magnesium and Ionized Calcium Concentrations
- Unit-Independent Reporting of Laboratory Test Results
- Analysis of Large and Small Samples of Biochemical and Clinical Data.
- Evaluation of Five Commercial Enzyme Immunoassays for the Detection of Human Cytomegalovirus-Specific IgM Antibodies in the Absence of a Commercially Available Gold Standard
- Alkaline Phosphatase Activity: New Assay for the Reflotron® System. Results of the Evaluation in Eight Clinical Laboratories
- Report on the 4th Estonian Summer School of Laboratory Medicine
- Laboratory-Related Measures of Patient Outcomes: An Introduction. Edited by Michael G. Bissell
- Report from the 50th Anniversary Congress of the Hungarian Society of Clinical Pathology and from the Executive Board Meeting of FESCC in Debrecen (Hungary)
- Report from the 8th Meeting of the Balkan Clinical Laboratory Federation
