Polymorphisms in the Apolipoprotein E Gene Regulatory Region in Relation to Coronary Heart Disease and Their Effect on Plasma Apolipoprotein E
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Rosa M. Corbo
Abstract
In a previous study which examined the distribution of apolipoprotein E genotypes and plasma levels in a sample of male coronary heart disease (CHD) patients and controls, we found a significant excess of the genotypes carrying APOE*4 allele in CHD men (18.2%) vs. controls (9.6%) and an association between the APOE*4 allele and the lowest concentrations of apoE. In the present investigation, we re-examined in the same samples two recently identified polymorphisms in the promoter region of APOE, −491A/T and −427T/C, which may alter the level of apoE expression. No differences in the distributions of the −491A/T genotypes and alleles were observed between cases and controls (−491*A = 0.760 and 0.757 respectively). Polymorphism −427T/C showed in CHD patients an excess of −427*C allele (patients vs. controls = 0.123 vs. 0.074) and corresponding genotypes that was marginally significant. Stratification of the samples according to the presence/absence of APOE*4 showed that the excess of the −427*C allele concerned only CHD patients not carrying APOE*4 allele (patients vs. controls = 0.133 vs. 0.061; p=0.017). This result suggests that the presence of −427*C allele could represent a risk for developing CHD in subjects with E2/E2, E3/E2, and E3/E3 genotypes. Studies carried out on patients with Alzheimer's disease demonstrated that −491A/T and −427T/C polymorphisms affect the level of plasma apoE. In the present study, carried out on CHD patients and controls, the genetic variation at −427 and −491 sites of the APOE regulatory region had no apparent effect on apoE plasma concentration.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Editorial
- Polymorphisms in the Apolipoprotein E Gene Regulatory Region in Relation to Coronary Heart Disease and Their Effect on Plasma Apolipoprotein E
- Further Characterization of Serum Amyloid A4 as a Minor Acute Phase Reactant and a Possible Nutritional Marker
- Determination of Human Angiotensin Converting Enzyme (ACE) Gene Polymorphisms in Erectile Dysfunction: Frequency Differences of ACE Gene Polymorphisms according to the Method of Analysis
- Simple Gas Chromatography Analysis of Faecal Butyrate: Application to Patients at Risk of Pouchitis
- Determination of Glycerol in Plasma by an Automated Enzymatic Spectrophotometric Procedure
- Clinical Applications of the 2nd Generation Assay for Anti-TSH Receptor Antibodies in Graves' Disease. Evaluation in Patients with Negative 1st Generation Test
- The Clinical Value of Microtransferrinuria and Microalbuminuria in the Prediction of Pre-eclampsia
- A Non-Icteric Cholecystectomized Patient with Recurrent Attacks of Right Epigastric Pain and Dilated Common Bile Duct – Do Liver Function Tests Predict Bile Duct Stones?
- Serum Lactate Dehydrogenase Isoenzyme 1 and Prediction of Death in Patients with Metastatic Testicular Germ Cell Tumors
- Annual Rhythmic and Non-Rhythmic Biological Variation of Magnesium and Ionized Calcium Concentrations
- Unit-Independent Reporting of Laboratory Test Results
- Analysis of Large and Small Samples of Biochemical and Clinical Data.
- Evaluation of Five Commercial Enzyme Immunoassays for the Detection of Human Cytomegalovirus-Specific IgM Antibodies in the Absence of a Commercially Available Gold Standard
- Alkaline Phosphatase Activity: New Assay for the Reflotron® System. Results of the Evaluation in Eight Clinical Laboratories
- Report on the 4th Estonian Summer School of Laboratory Medicine
- Laboratory-Related Measures of Patient Outcomes: An Introduction. Edited by Michael G. Bissell
- Report from the 50th Anniversary Congress of the Hungarian Society of Clinical Pathology and from the Executive Board Meeting of FESCC in Debrecen (Hungary)
- Report from the 8th Meeting of the Balkan Clinical Laboratory Federation
Articles in the same Issue
- Editorial
- Polymorphisms in the Apolipoprotein E Gene Regulatory Region in Relation to Coronary Heart Disease and Their Effect on Plasma Apolipoprotein E
- Further Characterization of Serum Amyloid A4 as a Minor Acute Phase Reactant and a Possible Nutritional Marker
- Determination of Human Angiotensin Converting Enzyme (ACE) Gene Polymorphisms in Erectile Dysfunction: Frequency Differences of ACE Gene Polymorphisms according to the Method of Analysis
- Simple Gas Chromatography Analysis of Faecal Butyrate: Application to Patients at Risk of Pouchitis
- Determination of Glycerol in Plasma by an Automated Enzymatic Spectrophotometric Procedure
- Clinical Applications of the 2nd Generation Assay for Anti-TSH Receptor Antibodies in Graves' Disease. Evaluation in Patients with Negative 1st Generation Test
- The Clinical Value of Microtransferrinuria and Microalbuminuria in the Prediction of Pre-eclampsia
- A Non-Icteric Cholecystectomized Patient with Recurrent Attacks of Right Epigastric Pain and Dilated Common Bile Duct – Do Liver Function Tests Predict Bile Duct Stones?
- Serum Lactate Dehydrogenase Isoenzyme 1 and Prediction of Death in Patients with Metastatic Testicular Germ Cell Tumors
- Annual Rhythmic and Non-Rhythmic Biological Variation of Magnesium and Ionized Calcium Concentrations
- Unit-Independent Reporting of Laboratory Test Results
- Analysis of Large and Small Samples of Biochemical and Clinical Data.
- Evaluation of Five Commercial Enzyme Immunoassays for the Detection of Human Cytomegalovirus-Specific IgM Antibodies in the Absence of a Commercially Available Gold Standard
- Alkaline Phosphatase Activity: New Assay for the Reflotron® System. Results of the Evaluation in Eight Clinical Laboratories
- Report on the 4th Estonian Summer School of Laboratory Medicine
- Laboratory-Related Measures of Patient Outcomes: An Introduction. Edited by Michael G. Bissell
- Report from the 50th Anniversary Congress of the Hungarian Society of Clinical Pathology and from the Executive Board Meeting of FESCC in Debrecen (Hungary)
- Report from the 8th Meeting of the Balkan Clinical Laboratory Federation
