Transient Nature of Interference Effects from Heterophile Antibodies: Examples of Interference with Cardiac Marker Measurements
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Steven C. Kazmierczak
Abstract
Two-site immunoassay methods have become the standard technique for measurement of a wide variety of drugs, hormones, and cell proteins. One limitation of these methods is their susceptibility to interference from heterophilic antibodies present in the sera of some patients. Human anti-murine antibodies represent a common heterophile antibody that can bind to mouse immunoglobulin and as well as to immunoglobulin from other species. While the mechanism of human anti-murine antibody interference has been well characterized, the time course over which this interference occurs and the susceptibility of different immunoassay procedures to human anti-murine antibody interference from patients with human anti-murine antibody have not been as well described. We report on the time course of interference in assays for cardiac markers for two patients with human anti-murine antibodies. We measured creatine kinase MB isoenzyme (CKMB) and troponins I and T using three different vendors' immunoassay procedures. Our results demonstrate that assay interference due to human anti-murine antibody interference is a transient phenomenon. In one of our patients, human anti-murine antibody interference appeared suddenly, peaked approximately 9 days following its appearance, and gradually resolved over the next 3 weeks. In addition, we found that immunoassay methods from different vendors can show highly variable interference effects when human anti-murine antibody-containing specimens are analyzed.
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- The Millenium 2000; Laboratory Medicine – from Where? to Where?
- Regulation of Lipid and Lipoprotein Metabolism by PPAR Activators
- Laboratory Screening for Erythropoietin Abuse in Sport: an Emerging Challenge
- Antioxidant Enzyme Activities and Total Nitrite/ Nitrate Levels in the Collar Model. Effect of Nicardipine
- Enzyme Immunoassays for Fragments (Epitopes) of Human Proatrial Natriuretic Peptides
- Transient Nature of Interference Effects from Heterophile Antibodies: Examples of Interference with Cardiac Marker Measurements
- Comparison of Procalcitonin, sCD14 and Interleukin-6 Values in Septic Patients
- Changes in Bone Turnover and Deoxypyridinoline Levels in Epileptic Patients
- Analytical Performance of the Fully Automated AxSYM(r) Estradiol Assay
- Impacts of Laboratory Methodology on Medical Thinking in the 19th Century
- Reference Intervals for Erythropoietin Measurement by the JCL EIA Assay Need to Be Determined Locally
- The Physical Basis of Biochemistry. By Peter R. Bergethon
- European Science. Science funding: the European dimension. A discussion meeting held at the Royal Society, Tuesday 17 March, 1998
- IFCC News
