Antioxidant Enzyme Activities and Total Nitrite/ Nitrate Levels in the Collar Model. Effect of Nicardipine
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Eser Yildirim Sözmen
Abstract
There is a large body of literature describing the causative role of oxidative stress mediated by increased levels of reactive oxygen species in the pathogenesis of cardiovascular disease such as atherosclerosis, hypertension, and restenosis after angioplasty. The positioning of a soft silicone collar around the rabbit carotid artery elicits intimal thickening. The findings from recent studies demonstrated that both intimal thickening and atherosclerosis lead to synthesis of inducible nitric oxide synthase, resulting in abundant amounts of nitric oxide. We investigated the effects of collaring and nicardipine treatment on the activities of antioxidant enzymes, superoxide dismutase and catalase, and total nitrite/nitrate levels, stable products of nitric oxide. Placing the collar increased the total nitrite/nitrate levels and decreased superoxide dismutase activity in collared arteries. Treatment with nicardipine (20 mg/kg/day, s.c.) prevented enhanced nitric oxide degradation without affecting superoxide dismutase and catalase activities. Our results suggest that enhanced nitric oxide production and superoxide anion are generated in response to the collaring, resulting in oxidative stress within the segment in this model.
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
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- The Millenium 2000; Laboratory Medicine – from Where? to Where?
- Regulation of Lipid and Lipoprotein Metabolism by PPAR Activators
- Laboratory Screening for Erythropoietin Abuse in Sport: an Emerging Challenge
- Antioxidant Enzyme Activities and Total Nitrite/ Nitrate Levels in the Collar Model. Effect of Nicardipine
- Enzyme Immunoassays for Fragments (Epitopes) of Human Proatrial Natriuretic Peptides
- Transient Nature of Interference Effects from Heterophile Antibodies: Examples of Interference with Cardiac Marker Measurements
- Comparison of Procalcitonin, sCD14 and Interleukin-6 Values in Septic Patients
- Changes in Bone Turnover and Deoxypyridinoline Levels in Epileptic Patients
- Analytical Performance of the Fully Automated AxSYM(r) Estradiol Assay
- Impacts of Laboratory Methodology on Medical Thinking in the 19th Century
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- The Physical Basis of Biochemistry. By Peter R. Bergethon
- European Science. Science funding: the European dimension. A discussion meeting held at the Royal Society, Tuesday 17 March, 1998
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