A New Liquid Homogeneous Assay for HDL Cholesterol Determination Evaluated in Seven Laboratories in Europe and the United States
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M. Nauck
Abstract
We evaluated a new liquid homogeneous assay for the direct measurement of high density lipoprotein cholesterol (HDL-C Plus) in seven laboratories. The assay includes two reagents which can be readily used in most available clinical chemistry analyzers.
The total CVs of the new method were below 4.6% and the bias in relation to the designated comparison method was below 3.9%. The total error ranged between 4 to 7%. HDL-C values determined by this method were in good agreement with those obtained by the old homogeneous assay using lyophilized reagents, and other homogeneous and precipitation assays (0.944 < r < 0.996). The assay was linear up to at least 3.89 mmol/l HDL-C.
Hemoglobin did not interfere, whereas in icteric samples slight deviations were observed. Lipemia up to 11.3 to 22.6 mmol/l triglycerides did not interfere with this homogeneous HDL-C assay. In samples of patients with paraproteinemia, discrepant results were seen. This liquid homogeneous HDL-C assay was easy to handle and produced similar results in all laboratories participating in this study. This method will enable clinical laboratories to reliably measure HDL-C for risk assessment of coronary heart disease.
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
Artikel in diesem Heft
- Author Index
- Contents
- Subject Index
- Strategies for Cardiac Marker Measurement
- Statistical Techniques for Evaluating the Diagnostic Utility of Laboratory Tests
- Magnesium in Disease: a Review with Special Emphasis on the Serum Ionized Magnesium
- Methods for Improving Clinical Trials
- Infrared Analysis of Urinary Stones: a Trial of Automated Identification
- Evaluation of a Direct α-Amylase Assay Using 2-Chloro-4-nitrophenyl-α-D-maltotrioside
- Accurate Platelet Counting in an Insidious Case of Pseudothrombocytopenia
- A New Liquid Homogeneous Assay for HDL Cholesterol Determination Evaluated in Seven Laboratories in Europe and the United States
- Tissue Release of Cardiac Markers: from Physiology to Clinical Applications
- The Specificity of Biochemical Markers of Cardiac Damage: a Problem Solved
- Biochemical Factors Influencing Measurement of Cardiac Troponin I in Serum
- The Sensitivity of Cardiac Markers: an Evidence-based Approach
- Risk Stratification and Therapeutic Decision Making in Patients with Acute Coronary Syndromes the Role of Cardiac Troponin T
- Cardiac Markers: Centralized or Decentralized Testing?
- EC4 European Syllabus for Post-Graduate Training in Clinical Chemistry. Version 2 – 1999
- Plasma Malondialdehyde and Obesity: Is there a Relationship?
- Reference Intervals: Are Interlaboratory Differences Appropriate?
- Lipoprotein Protocols. By J.M. Ordovas, editor
- Tietz Textbook of Clinical Chemistry. By C.A. Burtis and E.R. Ashwood, editors
Artikel in diesem Heft
- Author Index
- Contents
- Subject Index
- Strategies for Cardiac Marker Measurement
- Statistical Techniques for Evaluating the Diagnostic Utility of Laboratory Tests
- Magnesium in Disease: a Review with Special Emphasis on the Serum Ionized Magnesium
- Methods for Improving Clinical Trials
- Infrared Analysis of Urinary Stones: a Trial of Automated Identification
- Evaluation of a Direct α-Amylase Assay Using 2-Chloro-4-nitrophenyl-α-D-maltotrioside
- Accurate Platelet Counting in an Insidious Case of Pseudothrombocytopenia
- A New Liquid Homogeneous Assay for HDL Cholesterol Determination Evaluated in Seven Laboratories in Europe and the United States
- Tissue Release of Cardiac Markers: from Physiology to Clinical Applications
- The Specificity of Biochemical Markers of Cardiac Damage: a Problem Solved
- Biochemical Factors Influencing Measurement of Cardiac Troponin I in Serum
- The Sensitivity of Cardiac Markers: an Evidence-based Approach
- Risk Stratification and Therapeutic Decision Making in Patients with Acute Coronary Syndromes the Role of Cardiac Troponin T
- Cardiac Markers: Centralized or Decentralized Testing?
- EC4 European Syllabus for Post-Graduate Training in Clinical Chemistry. Version 2 – 1999
- Plasma Malondialdehyde and Obesity: Is there a Relationship?
- Reference Intervals: Are Interlaboratory Differences Appropriate?
- Lipoprotein Protocols. By J.M. Ordovas, editor
- Tietz Textbook of Clinical Chemistry. By C.A. Burtis and E.R. Ashwood, editors
