Semi-Automated Rapid Isoelectric Focusing of Apolipoproteins C from Human Plasma Using PhastSystem™ and Immunofixation
-
Bernd Noll
Abstract
Apolipoproteins (apo) C-I, C-II, and C-III play crucial roles in intravascular lipid metabolism. Whereas apo C-II is an obligate cofactor for lipoprotein lipase, apo C-III was shown to inhibit its action. Apo C-I can be a potent cofactor of human lecithin:cholesterol acyltransferase. Structural mutants and deficiencies of apo C-II lead to hypertriglyceridemia. A similar phenotype is associated with apo C-III mutants and is inducible by overexpression of human apo C-III in transgenic animals. No structural variant has so far been reported for apo C-I. The present paper describes a rapid semi-automated procedure for isoelectric focusing analysis of these C-apolipoproteins from whole plasma or serum and their visualization by immunofixation and silver staining. The procedure allows detection of charged variants of C-apolipoproteins. As applied to 295 patients with coronary heart disease and 85 controls, it also serves to detect deficiency syndromes of these apolipoproteins. The procedure provides reliable, easy and quick analysis of C-apolipoproteins applicable as a routine or screening procedure not restricted to specialized laboratories.
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Meetings and Awards
- Are Cardiac Troponins the Myocardial Markers for the New Millenium?
- Leukocyte Activation Markers in Clinical Practice
- Hepatitis C Virus-Specific DNA Sequences in Human DNA: Differentiation by Means of Restriction Enzyme Analysis at the DNA Level of Healthy, Anti-HCV-Negative Individuals
- Characterization of Monoclonal Antibodies to Human Protein 1/Clara Cell 10 Kilodalton Protein
- Rapid Analysis of Parathion in Biological Samples Using Headspace Solid-Phase Micro-Extraction (HS-SPME) and Gas Chromatography/Mass Spectrometry (GC/MS)
- Semi-Automated Rapid Isoelectric Focusing of Apolipoproteins C from Human Plasma Using PhastSystem™ and Immunofixation
- The c-erbB-2 Oncogene Amplification by Competitive PCR in Aneuploid Cell Clones Flow Sorted from Breast Cancer Samples
- The Index of Individuality Is Often a Misinterpreted Quantity Characteristic
- Assessment of Package Inserts for Diagnostic Kits
- External Quality Assessment in Clinical Chemistry: Review of the Situation in Croatia with Particular Reference to Equipment
- Point-of-Care Testing: the Views of the Working Group of the Dutch Association of Clinical Chemistry
- Preliminary Evaluation of the New Hematology Analyzer COULTER® GEN•S™ in a University Hospital
- Use of Biochemical Markers in Acute Coronary Syndromes. IFCC Scientific Division, Committee on Standardization of Markers of Cardiac Damage
- Oxidative Stress in Skeletal Muscle. A.E. Rezuick, L. Packer, C.K. Sen. J.O. Hollosey and M.J. Jackson, editors
- Handbook of Lipoprotein Testing. N. Rifai, C.G. Warnick and M.H. Dominiczak, editors
- Erratum: Matthias Oberhoffer, Heinz Vogelsang, Stefan Rußwurm, Thomas Hartung and Konrad Reinhart. Outcome Prediction by Traditional and New Markers of Inflammation in Patients with Sepsis
Articles in the same Issue
- Meetings and Awards
- Are Cardiac Troponins the Myocardial Markers for the New Millenium?
- Leukocyte Activation Markers in Clinical Practice
- Hepatitis C Virus-Specific DNA Sequences in Human DNA: Differentiation by Means of Restriction Enzyme Analysis at the DNA Level of Healthy, Anti-HCV-Negative Individuals
- Characterization of Monoclonal Antibodies to Human Protein 1/Clara Cell 10 Kilodalton Protein
- Rapid Analysis of Parathion in Biological Samples Using Headspace Solid-Phase Micro-Extraction (HS-SPME) and Gas Chromatography/Mass Spectrometry (GC/MS)
- Semi-Automated Rapid Isoelectric Focusing of Apolipoproteins C from Human Plasma Using PhastSystem™ and Immunofixation
- The c-erbB-2 Oncogene Amplification by Competitive PCR in Aneuploid Cell Clones Flow Sorted from Breast Cancer Samples
- The Index of Individuality Is Often a Misinterpreted Quantity Characteristic
- Assessment of Package Inserts for Diagnostic Kits
- External Quality Assessment in Clinical Chemistry: Review of the Situation in Croatia with Particular Reference to Equipment
- Point-of-Care Testing: the Views of the Working Group of the Dutch Association of Clinical Chemistry
- Preliminary Evaluation of the New Hematology Analyzer COULTER® GEN•S™ in a University Hospital
- Use of Biochemical Markers in Acute Coronary Syndromes. IFCC Scientific Division, Committee on Standardization of Markers of Cardiac Damage
- Oxidative Stress in Skeletal Muscle. A.E. Rezuick, L. Packer, C.K. Sen. J.O. Hollosey and M.J. Jackson, editors
- Handbook of Lipoprotein Testing. N. Rifai, C.G. Warnick and M.H. Dominiczak, editors
- Erratum: Matthias Oberhoffer, Heinz Vogelsang, Stefan Rußwurm, Thomas Hartung and Konrad Reinhart. Outcome Prediction by Traditional and New Markers of Inflammation in Patients with Sepsis
