Hepatitis C Virus-Specific DNA Sequences in Human DNA: Differentiation by Means of Restriction Enzyme Analysis at the DNA Level of Healthy, Anti-HCV-Negative Individuals
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Reinhard H. Dennin
Abstract
This study aimed to look for further HCV-specific sequences at the DNA level of healthy, HCV-negative individuals. Here, the sequence section from nt 57 to nt 328 within the 5′-NCR was assayed. Different combinations of primers were used in the nested PCR without a preceding reverse transcriptase step, resulting in fragments of the expected molecular weight size and also shorter and longer ones. It shows that the major part of the 5′-non-coding region (5′-NCR) of the hepatitis C virus genome is present in the DNA fraction from peripheral blood mononuclear cells (PBMCs) of healthy, anti-HCV-negative individuals tested. Furthermore we designed experiments to prove the specificity of these findings, by using restriction enzymes for digest assays of the target DNA before PCR (pre-PCR digest) and of the products after PCR (post-PCR digest). In conclusion, our study indicates that the main part of the internal ribosome entry site (IRES) structure of HCV at least is contained in the DNA of the individuals tested.
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Meetings and Awards
- Are Cardiac Troponins the Myocardial Markers for the New Millenium?
- Leukocyte Activation Markers in Clinical Practice
- Hepatitis C Virus-Specific DNA Sequences in Human DNA: Differentiation by Means of Restriction Enzyme Analysis at the DNA Level of Healthy, Anti-HCV-Negative Individuals
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- Semi-Automated Rapid Isoelectric Focusing of Apolipoproteins C from Human Plasma Using PhastSystem™ and Immunofixation
- The c-erbB-2 Oncogene Amplification by Competitive PCR in Aneuploid Cell Clones Flow Sorted from Breast Cancer Samples
- The Index of Individuality Is Often a Misinterpreted Quantity Characteristic
- Assessment of Package Inserts for Diagnostic Kits
- External Quality Assessment in Clinical Chemistry: Review of the Situation in Croatia with Particular Reference to Equipment
- Point-of-Care Testing: the Views of the Working Group of the Dutch Association of Clinical Chemistry
- Preliminary Evaluation of the New Hematology Analyzer COULTER® GEN•S™ in a University Hospital
- Use of Biochemical Markers in Acute Coronary Syndromes. IFCC Scientific Division, Committee on Standardization of Markers of Cardiac Damage
- Oxidative Stress in Skeletal Muscle. A.E. Rezuick, L. Packer, C.K. Sen. J.O. Hollosey and M.J. Jackson, editors
- Handbook of Lipoprotein Testing. N. Rifai, C.G. Warnick and M.H. Dominiczak, editors
- Erratum: Matthias Oberhoffer, Heinz Vogelsang, Stefan Rußwurm, Thomas Hartung and Konrad Reinhart. Outcome Prediction by Traditional and New Markers of Inflammation in Patients with Sepsis
