A New Liquid Homogeneous Assay for the Determination of HDL-Cholesterol
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Matthias Nauck
Abstract
We evaluated a new ready to use liquid assay for the homogeneous determination of HDL-cholesterol (HDL-C; Merck, Darmstadt, Germany) in comparison to phosphotungstic acid precipitation and a homogeneous assay, based on sulfated α-cyclodextrin and polyethylene glycol-modified enzymes (Roche Diagnostics/Boehringer Mannheim, Germany). The new liquid homogeneous HDL-C assay had inter-assay coefficients' of variation of less than 2.1 %. The method is linear up to at least 3.11 mmol/l HDL-C, but even at 4.40 mmol/l the deviation from the expected value is less than 5%.
Spinking experiments with low density lipoproteins and very low density lipoproteins proved that the new assay was specific for high density lipoproteins up to cholesterol associated with low density lipoproteins (LDL-C) and very low density lipoproteins (VLDL)-triglyceride concentrations of 18.13 and 22.60 mmol/l, respectively. Free fatty acids above 2 mmol/l did not interfere. Icteric samples with bilirubin concentrations between 170 and 400 μmol/l did not show any systematic deviation compared to the precipitation procedure. In addition, serum hemoglobin concentrations up to 7.0 mmol/l and ascorbic acid up to 3000 μmol/l did not interfere with the HDL-C assay. An intermethod comparison including 120 samples revealed good agreement of the liquid HDL-C assay and the precipitation procedure (y = 0.943x + 0.074 mmol/l; r = 0.992). The new homogeneous HDL-C assay is thus precise, comparable and robust. Due to its ease of handling this assay will significantly facilitate attempts to include the differentiation between HDL-C and LDL-C in the routine screening for cardiovascular risk factors and in the monitoring of lipid lowering therapy.
Copyright (c)1999 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Metrological Support of the Institute for Reference Materials and Measurements (IRMM) to Clinical Chemistry
- Web Applications for Total Quality Management
- Inflammation, the Acute Phase Response and Atherosclerosis
- Characterization of Two Distinct Mechanisms for Induction of Apoptosis in Human Vascular Endothelial Cells
- Lipid Peroxidation and Antioxidant Defenses in Cystic Fibrosis Patients
- Autoantibodies against Oxidised Low-Density Lipoprotein in Patients with Obstructive Sleep Apnoea
- Inter-α-Inhibitor (IαI) Concentration in Pig Plasma Is Independent of Acute-Phase Protein Response
- Advances in Reverse Transcription Polymerase Chain Reaction Analysis of Cellular mRNA Levels of Transforming Growth Factor-β1 by Capillary Electrophoresis with Laser-Induced Fluorescence Detection
- Determination of Ascorbic Acid in Plasma and Urine by High Performance Liquid Chromatography with Ultraviolet Detection
- A New Liquid Homogeneous Assay for the Determination of HDL-Cholesterol
- On the Standardization of Total Prostate-Specific Antigen: an Exercise with Two Reference Preparations
- Analytical Quality Specifications for Serum Lactate Dehydrogenase Isoenzyme 1 Based on Clinical Goals
- Physiological HEPES Buffer Proposed as a Calibrator for pH Measurement in Human Blood
- Towards Common Reference Intervals in Clinical Chemistry
- Evaluation of an Automated Method for Cytokine Measurement Using the Immulite® Immunoassay System
- Handbook of Interpretation of Diagnostic Tests by Jacques Wallach
- Assessment of a Major Academic Development Programme Linking European Union with Central and Eastern Europe
