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Determination of Ascorbic Acid in Plasma and Urine by High Performance Liquid Chromatography with Ultraviolet Detection
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Andreas Rümelin
Published/Copyright:
June 1, 2005
Abstract
A reliable simple reversed-phase liquid chromatographic method for the routine determination of ascorbic acid in plasma and urine with ultraviolet detection is described. This method enables the complete separation of the ascorbic acid peak from others with a recovery of above 95 % within 8 minutes. The method can be used for analysing multiple samples within a day. In addition, the storage conditions and stability of ascorbic acid in plasma and urine were investigated. Samples of plasma and urine can be stored on ice in darkness for at least 60 min without reduction of ascorbic acid concentration. Prepared samples can be stored in darkness at 4 °C for at least 120 min and in liquid nitrogen for 42 days.
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Published Online: 2005-06-01
Published in Print: 1999-05-01
Copyright (c)1999 by Walter de Gruyter GmbH & Co. KG
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- Web Applications for Total Quality Management
- Inflammation, the Acute Phase Response and Atherosclerosis
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