Polymorphonuclear Oxidative Burst after Helicobacter pylori Water Extract Stimulation Is not Influenced by the Cytotoxic Genotype but Indicates Infection and Gastritis Grade
-
Daniela Basso
Abstract
H. pylori-associated gastric mucosal inflammation is characterized by the presence of polymorphonuclear (PMN) leukocyte infiltrate, which is more severe when the infecting strain is cagA positive. After appropriate stimuli, such as bacterial products, PMN release large amounts of oxygen derived free radicals and proteases, to kill the bacterium. H. pylori seems to be particularly resistant to the oxidative machinery of PMN, which can in turn damage the host gastric mucosa. We evaluated peripheral PMN oxidative burst response after stimulation with water extracts from cagA positive (WEcagA+) or negative (WEcagA−) H. pylori strains in infected (n=31) and non-infected patients (n=32) in comparison with healthy controls (n=16); the influence of gastric mucosal inflammatory infiltrate and activity grade on PMN oxidative burst were also assessed. PMN oxidative burst was measured by FACS analysis. H. pylori water extracts were obtained from bacterial culture. H. pylori genotype was determined by means of the polymerase chain reaction. The PMN oxidative burst in H. pylori infected patients was significantly higher than that in H. pylori negative or healthy controls, no differences being found when the results following WEcagA+ and WEcagA-stimulation were compared. The difference in PMN oxidative burst obtained after WEcagA− and E. coli (standard stimulus for PMN oxidative burst) stimulation discriminated H. pylori infected from non-infected patients with a sensitivity of 90 % and a specificity of 97 %. The grade of PMN oxidative burst correlated with PMN infiltration grade of the gastric mucosa. Our findings allow to conclude that PMN oxidative burst activation by H. pylori WE is species-but not strain-correlated. PMN priming, probably consequent to the action of soluble mediators released by mononuclear cells, makes PMN hyper-responsive to H. pylori products, thus favoring the release in the gastric mucosa of infected patients of large amounts of oxygen-derived free radicals, which are not enough to eliminate the infection, but may contribute to damaging the gastric mucosa itself. Peripheral PMN oxidative burst response to H. pylori WE might furthermore be of help in diagnosing H. pylori infection.
Copyright (c)1999 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- IFCC/Beckman European Conference 1998 Frontiers in Molecular Basis of Disease: Inflammatory Diseases
- Frontiers in Molecular Basis of Disease: Inflammatory Diseases
- Mechanisms of Antigen Presentation
- How to Extinguish Lymphocyte Activation, Immunotyrosine-Based Inhibition Motif (ITIM)-Bearing Molecules a Solution?
- Association between Reduced Human Leukocyte Antigen (HLA)-DR Expression on Blood Monocytes and Increased Plasma Level of Interleukin-10 in Patients with Severe Burns
- Immunostimulatory CpG Motifs Trigger a T Helper-1 Immune Response to Human Immunodeficiency Virus Type-1 (HIV-1) gp160 Envelope Proteins
- Activation and Regulation of NFκB during Acute Inflammation
- CD 14++ Monocytes, CD14+/CD16+ Subset and Soluble CD14 as Biological Markers of Inflammatory Systemic Diseases and Monitoring Immuno-suppressive Therapy
- A Potential Role of an Intracellular Signaling Defect in Neutrophil Functional Abnormalities and Promotion of Tissue Damage in Patients with Localized Juvenile Periodontitis
- Polymorphonuclear Oxidative Burst after Helicobacter pylori Water Extract Stimulation Is not Influenced by the Cytotoxic Genotype but Indicates Infection and Gastritis Grade
- Flow Cytometry of Cerebrospinal Fluid (CSF) Lymphocytes: Alterations of Blood/CSF Ratios of Lymphocyte Subsets in Inflammation Disorders of Human Central Nervous System (CNS)
- Effects of Oxidized Low Density Lipoprotein, Lipid Mediators and Statins on Vascular Cell Interactions
- Mycophenolic Acid Influences T Helper 2 (Th2) Cytokine Induced Expression of Intercellular Cell Adhesion Molecule -1 (ICAM-1) on Human Endothelial Cells
- Alterations of Endothelial Nucleotide Levels by Mycophenolic Acid Result in Changes of Membrane Glycosylation and E-Selectin Expression
- C-Reactive Protein: Structural Biology and Host Defense Function
- A Novel Acute-Phase Marker: Lipopolysaccharide Binding Protein (LBP)
- Detection of Acute Phase Response and Infection. The Role of Procalcitonin and C-Reactive Protein
- Lactoferrin: A Multifunctional Glycoprotein Involved in the Modulation of the Inflammatory Process
- Annexin V and Phospholipid Metabolism
- Regulation of Leukocyte Trafficking by Lipoxins
- Lipoxin and Aspirin-Triggered 15-epi-Lipoxin Cellular Interactions Anti-Inflammatory Lipid Mediators
- Differentiation and Assessment of Cell Death
- Depending on Their Concentration Oxidized Low Density Lipoproteins Stimulate Extracellular Matrix Synthesis or Induce Apoptosis in Human Coronary Artery Smooth Muscle Cells
- Apheresis of Plasma Compounds as a Therapeutic Principle in Severe Sepsis and Multiorgan Dysfunction Syndrome
- Supportive Therapy of the Sepsis Syndrome
- Supplemental Immune Globulins in Sepsis
- Polymorphonuclear Granulocyte Stimulation by Cellulose-Based Hemodialysis Membranes
- Reduction of Circulating Cholesterol and Apolipoprotein Levels during Sepsis
- Outcome Prediction by Traditional and New Markers of Inflammation in Patients with Sepsis
- Isolation of Highly Enriched Human Monocytes from Ten ml Samples of Heparinised Whole Blood
- Endotoxin Adsorbant Based on Immobilized Human Serum Albumin
Articles in the same Issue
- IFCC/Beckman European Conference 1998 Frontiers in Molecular Basis of Disease: Inflammatory Diseases
- Frontiers in Molecular Basis of Disease: Inflammatory Diseases
- Mechanisms of Antigen Presentation
- How to Extinguish Lymphocyte Activation, Immunotyrosine-Based Inhibition Motif (ITIM)-Bearing Molecules a Solution?
- Association between Reduced Human Leukocyte Antigen (HLA)-DR Expression on Blood Monocytes and Increased Plasma Level of Interleukin-10 in Patients with Severe Burns
- Immunostimulatory CpG Motifs Trigger a T Helper-1 Immune Response to Human Immunodeficiency Virus Type-1 (HIV-1) gp160 Envelope Proteins
- Activation and Regulation of NFκB during Acute Inflammation
- CD 14++ Monocytes, CD14+/CD16+ Subset and Soluble CD14 as Biological Markers of Inflammatory Systemic Diseases and Monitoring Immuno-suppressive Therapy
- A Potential Role of an Intracellular Signaling Defect in Neutrophil Functional Abnormalities and Promotion of Tissue Damage in Patients with Localized Juvenile Periodontitis
- Polymorphonuclear Oxidative Burst after Helicobacter pylori Water Extract Stimulation Is not Influenced by the Cytotoxic Genotype but Indicates Infection and Gastritis Grade
- Flow Cytometry of Cerebrospinal Fluid (CSF) Lymphocytes: Alterations of Blood/CSF Ratios of Lymphocyte Subsets in Inflammation Disorders of Human Central Nervous System (CNS)
- Effects of Oxidized Low Density Lipoprotein, Lipid Mediators and Statins on Vascular Cell Interactions
- Mycophenolic Acid Influences T Helper 2 (Th2) Cytokine Induced Expression of Intercellular Cell Adhesion Molecule -1 (ICAM-1) on Human Endothelial Cells
- Alterations of Endothelial Nucleotide Levels by Mycophenolic Acid Result in Changes of Membrane Glycosylation and E-Selectin Expression
- C-Reactive Protein: Structural Biology and Host Defense Function
- A Novel Acute-Phase Marker: Lipopolysaccharide Binding Protein (LBP)
- Detection of Acute Phase Response and Infection. The Role of Procalcitonin and C-Reactive Protein
- Lactoferrin: A Multifunctional Glycoprotein Involved in the Modulation of the Inflammatory Process
- Annexin V and Phospholipid Metabolism
- Regulation of Leukocyte Trafficking by Lipoxins
- Lipoxin and Aspirin-Triggered 15-epi-Lipoxin Cellular Interactions Anti-Inflammatory Lipid Mediators
- Differentiation and Assessment of Cell Death
- Depending on Their Concentration Oxidized Low Density Lipoproteins Stimulate Extracellular Matrix Synthesis or Induce Apoptosis in Human Coronary Artery Smooth Muscle Cells
- Apheresis of Plasma Compounds as a Therapeutic Principle in Severe Sepsis and Multiorgan Dysfunction Syndrome
- Supportive Therapy of the Sepsis Syndrome
- Supplemental Immune Globulins in Sepsis
- Polymorphonuclear Granulocyte Stimulation by Cellulose-Based Hemodialysis Membranes
- Reduction of Circulating Cholesterol and Apolipoprotein Levels during Sepsis
- Outcome Prediction by Traditional and New Markers of Inflammation in Patients with Sepsis
- Isolation of Highly Enriched Human Monocytes from Ten ml Samples of Heparinised Whole Blood
- Endotoxin Adsorbant Based on Immobilized Human Serum Albumin
