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Rapid Electrophoretic Separation of Pre-ß-Migrating High Density Lipoproteins Using Automated PhastSystem(tm): Application to Analysis of Lecitihin: Cholesterol Acyltransferase-Deficient Plasma
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Youssef Saïdi
Published/Copyright:
June 1, 2005
Abstract
To identify pre-β-high density lipoproteins, a rapid two-dimensional separation by electrophoresis (1 hour 30 minutes) was performed on an automated Phast System™. This procedure used commercially available polyacrylamide gradient gels (4–15 %) and allows sensitive and reproducible results. Pre-β-1- and pre-β-2-high density lipoproteins were clearly identified by this method. In addition, our procedure was successfully applied to diagnosis of a patient with familial lecithin: cholesterol acyltransferase deficiency, characterized by the absence of α-high density lipoproteins.
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Published Online: 2005-06-01
Published in Print: 1998-06-01
Walter de Gruyter GmbH & Co. KG
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- Progress in Immunoassay Technology
- Standardization of Steroid Immunoassays – In Theory an Easy Task
- Thermodynamic Parameters in Immunoassay
- Engineering of an Anti-Steroid Antibody: Amino Acid Substitutions Change Antibody Fine Specificity from Cortisol Estradiol
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- Remodeling of Glycoprotein and Carbohydrate Antigens
- Strongly Enhanced Serum Levels of Vascular Endothelial Growth Factor (VEGF) after Poly-trauma and Burn
- Rapid Electrophoretic Separation of Pre-ß-Migrating High Density Lipoproteins Using Automated PhastSystem(tm): Application to Analysis of Lecitihin: Cholesterol Acyltransferase-Deficient Plasma
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