Nitrate reductase in the marine macroalga Kappaphycus alvarezii (Rhodophyta): oscillation due to the protein level
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Malena Granbom
Abstract
Nitrate reductase (NR; EC 1.6.6.1) of the marine red macroalga Kappaphycus alvarezii was examined in algae maintained under L:D and constant light conditions. Undenaturated protein had a molecular mass of about 210 kD (±23 kD); based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting techniques, the enzyme appears to be composed of two possibly identical subunits of 100 kD. The NR of K. alvarezii was recognized by 10 out of 17 monoclonal antibodies raised against the NR from the red alga Porphyra yezoensis. Basal parts of the algal thallus had higher NR protein content, although the highest activity of NR has been located previously in the apical thallus parts, suggesting a post-translational regulation. The cellular expression of NR exhibits a daily rhythm. In extracts of algae grown under either constant light or a light:dark cycle, staining with antibodies NR 6 and NR 10 showed that the amount of protein varied by a factor of about 2, with the maximum occurring in the early day phase.
©2007 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Spatial and temporal changes in non-structural carbohydrate reserves in eelgrass (Zostera marina L.) in Danish coastal waters
- Diversity, abundance and distribution of macroalgae at Sirinart Marine National Park, Phuket Province, Thailand
- Vertical distribution of spores of blade-forming Sarcothalia crispata (Gigartinaceae) and crustose corallines (Corallinaceae) in the water column
- Nitrate reductase in the marine macroalga Kappaphycus alvarezii (Rhodophyta): oscillation due to the protein level
- Filament formation and differentiation in seven species of red algae
- New records of benthic marine algae from the Canary Islands (eastern Atlantic Ocean): morphology, taxonomy and distribution
- Notes on the marine algae of the Bermudas. 8. Further additions to the flora, including Griffithsia aestivana sp. nov. (Ceramiaceae, Rhodophyta) and an update on the alien Cystoseira compressa (Sargassaceae, Heterokontophyta)
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