Purification and some properties of a d-fructose-1,6-bisphosphate aldolase from the red alga, Gracilaria chorda Holmes
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Hirotaka Kakita
Abstract
D-Fructose-1,6-bisphosphate aldolase (0.042 mg) was purified from a red alga, Gracilaria chorda, by ammonium sulfate precipitation, followed by ion exchange and hydrophobic interaction chromatographies. The enzyme was purified 87-fold resulting in a final specific activity of 10.7 units/mg and a homogeneous appearance on electrophoresis. By SDS-PAGE analysis of the enzyme, two protein bands corresponding to molecular masses of 45 kDa and 39 kDa were observed. The enzymic activity disappeared in the presence of 0.5 mM EDTA, but was restored to 109% of the original untreated activity by the addition of 1 mM ZnSO4 subsequent to EDTA treatment. The enzyme was activated up to 5.5 times the original untreated activity by the addition of 500 mM KCl, and had a narrow optimum pH around 7.2. On the basis of these results, the G. chorda D-fructose-1,6-bisphosphate aldolase was characterized as a class II aldolase.
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Articles in the same Issue
- Sulfur cycling and sulfide intrusion in mixed Southeast Asian tropical seagrass meadows
- Variability of Ruppia cirrhosa in two coastal lagoons with differing anthropogenic stresses
- A re-evaluation of the taxonomic status of Halophila euphlebia Makino (Hydrocharitaceae) based on morphological features and ITS sequence data
- Ganonema vermiculare sp. nov. (Liagoraceae, Rhodophyta), a new species from Puerto Rico, Caribbean Sea
- Antithamnionella multiglandulosa sp. nov. (Rhodophyta, Ceramiaceae) from the Atlantic Iberian Peninsula
- Changes in the benthic algal flora of Linosa Island (Straits of Sicily, Mediterranean Sea)
- Seasonal and spatial dynamics of Noctiluca scintillans in a semi-enclosed bay in the northeastern part of Hong Kong
- Taxonomic appraisal of Melosira arctica Dickie and description of a new variety (Bacillariophyta)
- Changes in growth, chlorophyll fluorescence and fatty acid composition with culture age in batch cultures of Phaeodactylum tricornutum and Chaetoceros muelleri (Bacillariophyceae)
- Purification and some properties of a d-fructose-1,6-bisphosphate aldolase from the red alga, Gracilaria chorda Holmes
- Biomass and docosahexaenoic acid formation by Schizochytrium mangrovei Sk-02 at low salt concentrations