Type II DNA Topoisomerase (Top2) as Promising Molecular Marker for Phylogenetic Analysis in Rhodophyta
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K. Shimomura
Abstract
Taxonomic uncertainty still exists in the Rhodophyta, and more suitable molecular markers for their phylogeny are required to establish conclusively their taxonomic relationship. In this paper, we describe sequencing, characterisation of partial nucleotide sequence of the TOP2 and phylogenetic analysis of the Top2, which is translated from the TOP2 in Porphyra yezoensis. The length of the partial nucleotide sequence of TOP2 was 1,208 bp and the predicted amino acid sequence was 402 amino acids. The present sequences matched to known TOP2 or Top2 sequences in the public databases and consensus sequences for the motifs [Top2 signature, ATP/GTPbinding site motif A (P-loop), etc.] are indicated in the amino acid sequences. In our phylogenetic trees, the Rhodophyta occupied an independent position within the crown eukaryotes. Thus, we can advocate that we succeeded in the determination of TOP2 and Top2 sequences of P. yezoensis andTop2may be a promising marker for phylogenetic analysis of the Rhodophyta. This is the first report to deal with Top2 from the Rhodophyta.
Copyright © 2002 by Walter de Gruyter GmbH & Co. KG
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- Meetings
Articles in the same Issue
- Evaluation of the Structure of the Polysaccharides from Chondria macrocarpa and Ceramium rubrum as Determined by 13C NMR Spectroscopy
- Desorption of Cadmium and the Reuse of Brown Seaweed Derived Products as Biosorbents
- Fatty Acids of Marine Algae from the Pacific Coast of North California
- Seasonal Variations in Carbon and Nitrogen Constituents in Eelgrass (Zostera marina L.) as Influenced by Increased Temperature and Water-Column Nitrate
- Sublittoral and Deep-Water Red and Brown Algae New from the Canary Islands
- Physiological Studies of Subtropical Mangrove Thraustochytrids
- Seasonal Lipid Composition in Macroalgae of the Northeastern Pacific Ocean
- First Report of Polysiphonia morrowii Harvey (Ceramiales, Rhodophyta) in the Mediterranean Sea
- Different Effects of Caulerpa racemosa on Two Co-occurring Seagrasses in the Mediterranean
- Dipterocladia arabiensis sp. nov. (Dasyaceae, Rhodophyta) from the Sultanate of Oman
- Type II DNA Topoisomerase (Top2) as Promising Molecular Marker for Phylogenetic Analysis in Rhodophyta
- Meetings
