Generation of synthetic RNA-based thermosensors
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Abstract
Structured RNAs with fundamental sensory and regulatory potential have been discovered in all kingdoms of life. Bacterial RNA thermometers are located in the 5′-untranslated region of certain heat shock and virulence genes. They regulate translation by masking the Shine-Dalgarno sequence in a temperature-dependent manner. To engineer RNA-based thermosensors, we used a combination of computer-based rational design and in vivo screening. After only two rounds of selection, several RNA thermometers that are at least as efficient as natural thermometers were obtained. Structure probing experiments revealed temperature-dependent conformational changes in these translational control elements. Our study demonstrates that temperature-controlled RNA elements can be designed by a simple combined computational and experimental approach.
©2008 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Guest Editorial
- Highlight – Viruses and Signaling
- Highlight: Viruses and Signaling
- HIV-1 at the immunological and T-lymphocytic virological synapse
- Pursuing different ‘TRADDes’: TRADD signaling induced by TNF-receptor 1 and the Epstein-Barr virus oncoprotein LMP1
- RNA viruses and the mitogenic Raf/MEK/ERK signal transduction cascade
- Hepatitis C virus (HCV) employs multiple strategies to subvert the host innate antiviral response
- Sabotage of antiviral signaling and effectors by influenza viruses
- Influenza viruses and the NF-κB signaling pathway – towards a novel concept of antiviral therapy
- Genes and Nucleic Acids
- Specific inhibition of transcriptional activity of the constitutive androstane receptor (CAR) by the splicing factor SF3a3
- Generation of synthetic RNA-based thermosensors
- Molecular Medicine
- Impaired synthesis of heme oxygenase-1 in Fanconi anemia cells can be rescued by transfection of Fanconi wild-type cDNA
- Cell Biology and Signaling
- Effects of the Cdc2-like kinase-family and DNA topoisomerase I on the alternative splicing of eNOS in TNF-α-stimulated human endothelial cells
- Proteolysis
- Cytotoxic and peptidase inhibitory activities of selected non-hepatotoxic cyclic peptides from cyanobacteria
