Glucocorticoid receptor-mediated expression of kallikrein 10 in human breast cancer cell lines
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Vathany Kulasingam
Abstract
Using the breast cell lines MCF-10A, MDA-MB-468 and T-47D, we investigated the role of various glucocorticoids in regulating human kallikrein 10 expression. We found that increased concentrations of glucocorticoids decreased KLK10 expression in MCF-10A and increased KLK10 expression in MDA-MB-468 and T-47D cells. Stimulation of the cell lines using other steroid hormones did not yield any difference in KLK10 expression in MCF-10A and MDA-MB-468 cells, suggesting that regulation of KLK10 occurs primarily through glucocorticoids. However, T-47D cells expressed higher levels of KLK10 upon dihydrotestosterone stimulation. Blocking the glucocorticoid receptor (GR) demonstrated that the mechanisms of induction and repression are different in the three cell lines studied. Taken together, our results suggest an alternative mode of KLK10 regulation – by glucocorticoids via GR-dependent mechanisms.
©2007 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Selenoproteins – biochemistry and clinical relevance
- Selenium in mammalian spermiogenesis
- Selenium in chemistry and biochemistry in comparison to sulfur
- Molecular biology of glutathione peroxidase 4: from genomic structure to developmental expression and neural function
- Physiological role of phospholipid hydroperoxide glutathione peroxidase in mammals
- Activation of the glutathione peroxidase 2 (GPx2) promoter by β-catenin
- Effect of age on sexually dimorphic selenoprotein expression in mice
- Post-translational processing of selenoprotein P: implications of glycosylation for its utilisation by target cells
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- Towards understanding selenocysteine incorporation into bacterial proteins
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- Glucocorticoid receptor-mediated expression of kallikrein 10 in human breast cancer cell lines
