Startseite Activation processing of cathepsin H impairs recognition by its propeptide
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Activation processing of cathepsin H impairs recognition by its propeptide

  • Martin Horn , Lucie Dolečková-Marešová , Lubomír Rulíšek , Martin Máša , Olga Vasiljeva , Boris Turk , Tudeviin Gan-Erdene , Miroslav Baudyš und Michael Mareš
Veröffentlicht/Copyright: 9. September 2005

Abstract

Free propeptides are known to function as inhibitors of the parental mature cysteine cathepsins. This general rule, however, does not apply to the aminopeptidase cathepsin H. Screening of propeptide fragments for their inhibitory potency revealed no significant effect on the native mature cathepsin H. On the other hand, inhibitory interaction was established with recombinant cathepsin H that displays endopeptidase activity due to a lack of the mini-chain. This finding suggests that the propeptide-binding region is structurally rearranged during maturation processing and mini-chain formation, which impairs the effective recognition of mature cathepsin H by its own propeptide.


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Received: 2005-5-11
Accepted: 2005-6-22
Published Online: 2005-9-9
Published in Print: 2005-9-1

©2005 by Walter de Gruyter Berlin New York

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