The NC1 dimer of human placental basement membrane collagen IV: does a covalent crosslink exist?
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Manuel E. Than
Abstract
Triple-helical collagen IV protomers associate through their N- and C-termini, forming a three-dimensional network that provides basement membranes with mechanical strength. Within this network, the C-terminal non-collagenous (NC1) domains form tight dimeric junctions. Crystallographic analyses of isolated NC1 domains show two trimeric cap-like structures interacting via a large interface. Previously, for NC1 from human placenta type-IV collagen we described covalent α1-α1 and α2-α2 crosslinks between Met93 and Lys211 of opposing α1(IV) and α2(IV) NC1-chains, which further stabilize this interface and explain the occurrence of reduction-insensitive NC1-chain dimers. However, their existence was recently questioned, and we therefore analyzed NC1-domain dimers in more detail by biochemical and protein crystallographic methods. Short-exposure diffraction data show a clear electron density cross-connecting the respective residues, which gradually disappears with prolonged crystal irradiation. Sequence analyses of isolated tryptic peptides derived from denatured NC1 monomers and dimers indicate that only the dimers, but not the monomers, yield these chemically labile cross-linked peptides. These data clearly demonstrate the presence of reduction-resistant, but chemically and radiation-sensitive covalent crosslinks between the side chains of Met93 and Lys211 in human placenta type-IV collagen.
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©2005 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Highlight: Molecular Machines
- Splicing of a rare class of introns by the U12-dependent spliceosome
- Proteasome-associated proteins: regulation of a proteolytic machine
- Novel insights into the mechanism of chaperone-assisted protein disaggregation
- Dynamic chromatin: concerted nucleosome remodelling and acetylation
- Efficient production of native actin upon translation in a bacterial lysate supplemented with the eukaryotic chaperonin TRiC
- The NC1 dimer of human placental basement membrane collagen IV: does a covalent crosslink exist?
- Intact growth factors are conserved in the extracellular matrix of ancient human bone and teeth: a storehouse for the study of human evolution in health and disease
- Differential accumulation of plastid preprotein translocon components during spruce (Picea abies L. Karst.) needle development
- Hypothyroidism induces expression of the peptide transporter PEPT2
- The precursor of secreted aspartic proteinase Sapp1p from Candida parapsilosis can be activated both autocatalytically and by a membrane-bound processing proteinase
- Effects of elastase and cathepsin G on the levels of membrane and soluble TNFα
- Isolation and structural characterisation of a novel 13-amino acid insulin-releasing peptide from the skin secretion of Agalychnis calcarifer
