Induction of MMP-10 and MMP-1 in a squamous cell carcinoma cell line by ultraviolet radiation
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M. C. Ramos
Abstract
Ultraviolet radiation may cause non-melanoma skin cancer by genetic and epigenetic events. In this study, we investigated in a squamous cell carcinoma cell line, SCL-1, whether UV irradiation modulates the expression of matrix metalloproteinases, known to be involved in tumor progression and metastasis by degradation of extracellular matrix components. UVA or UVB irradiation of SCL-1 resulted in a rapid transcriptional upregulation and increased secretion of two members of the matrix metalloproteinase family, MMP-10 (stromelysin-2) and MMP-1 (interstitial collagenase). The increase in MMP-10 steadystate mRNA levels was detected 1 hour after UVA and 4 h after UVB irradiation, whereas MMP-1 was upregulated 4 h after UVA and 16 h after UVB irradiation of tumor cells. UVinduced phosphorylation of extracellular regulated kinases (ERK-1/2) and p38 stress kinase and increased binding of AP-1 transcription factor preceded the rapid stimulation of MMPs in SCL-1 cells. Incubation of cells with the MEK1/2 inhibitor U0126 or the p38 inhibitor SB202190 abolished the UVA and UVB mediated induction of MMP-1 and MMP-10. In conclusion, this study shows that UV irradiation of squamous cell carcinoma results in a rapid upregulation of MMPs. Our results suggest that the time course of induction of target genes, like MMPs, differs between cell types depending on the stimulus.
Copyright © 2004 by Walter de Gruyter GmbH & Co. KG
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Artikel in diesem Heft
- The chemistry of nitrosative stress induced by nitric oxide and reactive nitrogen oxide species. Putting perspective on stressful biological situations
- Til cell death do us part: nitric oxide and mechanisms of hepatotoxicity
- Singlet oxygen-mediated damage to cellular DNA determined by the comet assay associated with DNA repair enzymes
- Functional studies on the ligand-binding domain of Ultraspiracle from Drosophila melanogaster
- Structural destabilization of the recombinant thermophilic TthL11 ribosomal protein by a single amino acid substitution
- Relevance of the kinetic equilibrium of forces to the control of the cell cycle by Ras proteins
- N-linked glycosylation of the human bradykinin B2 receptor is required for optimal cell-surface expression and coupling
- Identification of sialic acids on Leishmania donovani amastigotes
- Induction of lipoprotein lipase gene expression in Chlamydia pneumoniae-infected macrophages is dependent on Ca2+ signaling events
- Induction of MMP-10 and MMP-1 in a squamous cell carcinoma cell line by ultraviolet radiation
- Monitoring human parvovirus B19 virus-like particles and antibody complexes in solution by fluorescence correlation spectroscopy
- In vitro folding and characterization of the p53 DNA binding domain
