Article
Licensed
Unlicensed
Requires Authentication
The PPIase Active Site of Legionella pneumophila Mip Protein Is Involved in the Infection of Eukaryotic Host Cells
-
J.H. Helbig
Published/Copyright:
June 1, 2005
Abstract
We analysed eight monoclonal antibodies (mAbs) directed against the Mip (macrophage infectivity potentiator) protein, a virulence factor of the intracellular pathogen Legionella pneumophila. Mip belongs to the FK506-binding proteins (FKBPs) and exhibits peptidyl prolyl cis/trans isomerase (PPIase) activity. Five of the mAbs recognised epitopes in the Cterminal, FKBP-homologous domain of Mip, which is highly conserved among all Legionella species. Upon immunological binding to Mip, all but one of these mAbs caused inhibition of the PPIase activity in vitro. mAb binding to the N-terminal domain of Mip did not influence its enzymatic activity. All but one of the PPIase inhibiting mAbs were able to significantly inhibit the early establishment and initiation of an intracellular infection of the bacteria in Acanthamoeba castellanii, the natural host, and in the human phagocytic cell line U937. These data demonstrate for the first time that for the virulence-enhancing property of the L. pneumophila Mip protein, an intact active site of the enzyme is an essential requirement.
:
Published Online: 2005-06-01
Published in Print: 2003-01-27
Copyright © 2003 by Walter de Gruyter GmbH & Co. KG
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Conserved Repressive Regulation of Connective Tissue Growth Factor/Hypertrophic Chondrocyte-Specific Gene 24 (ctgf/hcs24) Enabled by Different Elements and Factors among Vertebrate Species
- 3’UTRs of Glutathione Peroxidases Differentially Affect Selenium-Dependent mRNA Stability and Selenocysteine Incorporation Efficiency
- DNA Polymerase β Gene Expression: The Promoter Activator CREB-1 Is Upregulated in Chinese Hamster Ovary Cells by DNA Alkylating Agent-Induced Stress
- Identification and Characterization of Proteins That Selectively Interact with Isoforms of the mRNA Binding Protein AUF1 (hnRNP D)
- Hepatitis B Virus HBx Peptide 116–138 and Proteasome Activator PA28 Compete for Binding to the Proteasome α4/MC6 Subunit
- Different Sensitivities of Mutants and Chimeric Forms of Human Muscle and Liver Fructose- 1,6-Bisphosphatases towards AMP
- Purification of Drosophila melanogaster Ultraspiracle Protein and Analysis of Its A/B Region-Dependent Dimerization Behavior in vitro
- MSP-1 Malaria Pseudopeptide Analogs: Biological and Immunological Significance and Three-Dimensional Structure
- Caenorhabditis elegans S-Adenosylmethionine Decarboxylase Is Highly Stimulated by Putrescine But Exhibits a Low Specificity for Activator Binding
- Nuclear Magnetic Resonance Studies on the pKa Values and Interaction of Ionizable Groups in Bromelain Inhibitor VI from Pineapple Stem
- Characterization of the Ligand-Binding Domain of the Ecdysteroid Receptor from Drosophila Melanogaster
- Biochemical Characterization of β2-Adrenergic Receptor Dimers and Oligomers
- The PPIase Active Site of Legionella pneumophila Mip Protein Is Involved in the Infection of Eukaryotic Host Cells
- Identification by Phage Display of the Linear Continuous MRPr1 Epitope in the Multidrug Resistance-Associated Protein (MRP1)
- Thiol Antioxidant Reversal of Pyrrolidine Dithiocarbamate-Induced Reciprocal Regulation of AP-1 and NF-κB
- Interaction between Leptin and Insulin Signaling Pathways Differentially Affects JAK-STAT and PI 3-Kinase-Mediated Signaling in Rat Liver
- Heat Shock Treatment Decreases E2F1-DNA Binding and E2F1 Levels in Human A549 Cells
- The Cellular Control Enzyme PolyADP Ribosyl Transferase Is Eliminated in Cultured Fanconi Anemia Fibroblasts at Confluency
- ‘Piggy-Back’ Transport of Xenopus Hyaluronan Synthase (XHAS1) via the Secretory Pathway to the Plasma Membrane
- Internalisation and Degradation of Histatin 5 by Candida albicans
Articles in the same Issue
- Conserved Repressive Regulation of Connective Tissue Growth Factor/Hypertrophic Chondrocyte-Specific Gene 24 (ctgf/hcs24) Enabled by Different Elements and Factors among Vertebrate Species
- 3’UTRs of Glutathione Peroxidases Differentially Affect Selenium-Dependent mRNA Stability and Selenocysteine Incorporation Efficiency
- DNA Polymerase β Gene Expression: The Promoter Activator CREB-1 Is Upregulated in Chinese Hamster Ovary Cells by DNA Alkylating Agent-Induced Stress
- Identification and Characterization of Proteins That Selectively Interact with Isoforms of the mRNA Binding Protein AUF1 (hnRNP D)
- Hepatitis B Virus HBx Peptide 116–138 and Proteasome Activator PA28 Compete for Binding to the Proteasome α4/MC6 Subunit
- Different Sensitivities of Mutants and Chimeric Forms of Human Muscle and Liver Fructose- 1,6-Bisphosphatases towards AMP
- Purification of Drosophila melanogaster Ultraspiracle Protein and Analysis of Its A/B Region-Dependent Dimerization Behavior in vitro
- MSP-1 Malaria Pseudopeptide Analogs: Biological and Immunological Significance and Three-Dimensional Structure
- Caenorhabditis elegans S-Adenosylmethionine Decarboxylase Is Highly Stimulated by Putrescine But Exhibits a Low Specificity for Activator Binding
- Nuclear Magnetic Resonance Studies on the pKa Values and Interaction of Ionizable Groups in Bromelain Inhibitor VI from Pineapple Stem
- Characterization of the Ligand-Binding Domain of the Ecdysteroid Receptor from Drosophila Melanogaster
- Biochemical Characterization of β2-Adrenergic Receptor Dimers and Oligomers
- The PPIase Active Site of Legionella pneumophila Mip Protein Is Involved in the Infection of Eukaryotic Host Cells
- Identification by Phage Display of the Linear Continuous MRPr1 Epitope in the Multidrug Resistance-Associated Protein (MRP1)
- Thiol Antioxidant Reversal of Pyrrolidine Dithiocarbamate-Induced Reciprocal Regulation of AP-1 and NF-κB
- Interaction between Leptin and Insulin Signaling Pathways Differentially Affects JAK-STAT and PI 3-Kinase-Mediated Signaling in Rat Liver
- Heat Shock Treatment Decreases E2F1-DNA Binding and E2F1 Levels in Human A549 Cells
- The Cellular Control Enzyme PolyADP Ribosyl Transferase Is Eliminated in Cultured Fanconi Anemia Fibroblasts at Confluency
- ‘Piggy-Back’ Transport of Xenopus Hyaluronan Synthase (XHAS1) via the Secretory Pathway to the Plasma Membrane
- Internalisation and Degradation of Histatin 5 by Candida albicans
