Octadecanoid and Jasmonate Signaling in Tomato (Lycopersicon esculentum Mill.) Leaves: Endogenous Jasmonates Do Not Induce Jasmonate Biosynthesis
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O. Miersch
Abstract
Jasmonates and their precursors, the octadecanoids, are signals in stress-induced alteration of gene expression. Several mRNAs coding for enzymes of jasmonic acid (JA) biosynthesis are up-regulated upon JA treatment or endogenous increase of the JA level. Here we investigated the positive feedback of endogenous JA on JA formation, as well as its β-oxidation steps. JA-responsive gene expression was recorded in terms of proteinase inhibitor2 (pin2) mRNA accumulation. JA formed upon treatment of tomato (Lycopersicon esculentum cv. Moneymaker) leaves with JA derivatives carrying different lengths of the carboxylic acid side chain was quantified by gas chromatography-mass spectrometry (GC-MS). The data revealed that β-oxidation of the side chain occurs up to a butyric acid moiety. The amount of JA formed from side-chain modified JA derivatives correlated with pin2-mRNA accumulation. JA derivatives with a carboxylic side chain of 3, 5 or 7 carbon atoms were unable to form JA and to express on pin2, whereas evennumbered derivatives were active.
After treatment of tomato leaves with (10-2H)-(–)-12-oxophytoenoic acid, (4-2H)-(–)-JA and its methyl ester were formed and could be quantified separately from the endogenously nonlabeled JA pool by GC-MS analysis via isotopic discrimination. The level of 8 nmol per g fresh weight JA and its methyl ester originated exclusively from labeled 12-oxophytoenic acid. This and further data indicate that endogenous synthesis of the JA precursor 12-oxophytodienoic acid, as well as of JA and its methyl ester, are not induced in tomato leaves, suggesting that positive feedback in JA biosynthesis does not function in vivo.
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
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- The Hepta-? -Glucoside Elicitor-Binding Proteins from Legumes Represent a Putative Receptor Family
- Octadecanoid and Jasmonate Signaling in Tomato (Lycopersicon esculentum Mill.) Leaves: Endogenous Jasmonates Do Not Induce Jasmonate Biosynthesis
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- Identification of the Arabidopsis thaliana Flavonoid 3'-Hydroxylase Gene and Functional Expression of the Encoded P450 Enzyme
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Articles in the same Issue
- To Our Authors, Readers and Subscribers
- Highlight: Plant Biological Chemistry
- The Structural Properties of Plant Peroxisomes and Their Metabolic Significance
- Active Oxygen Species as Mediators of Plant Immunity: Three Case Studies
- Post-Transcriptional Regulation of Phenylalanine Ammonia-Lyase Expression in Tobacco Following Recovery from Gene Silencing
- Two Differentially Regulated Class II Chitinases from Parsley
- IAA-Synthase, an Enzyme Complex from Arabidopsis thaliana Catalyzing the Formation of Indole-3-Acetic Acid from (S)-Tryptophan
- Topology Studies of the Chloroplast Protein Import Channel Toc75
- Plant Methionine Synthase: New Insights into Properties and Expression
- The Hepta-? -Glucoside Elicitor-Binding Proteins from Legumes Represent a Putative Receptor Family
- Octadecanoid and Jasmonate Signaling in Tomato (Lycopersicon esculentum Mill.) Leaves: Endogenous Jasmonates Do Not Induce Jasmonate Biosynthesis
- Metabolic Activity Decreases as an Adaptive Response to Low Internal Oxygen in Growing Potato Tubers
- Structure-Activity Relationships of Synthetic Analogs of Jasmonic Acid and Coronatine on Induction of Benzophenanthridine Alkaloid Accumulation in Eschscholzia californica Cell Cultures
- Identification of the Arabidopsis thaliana Flavonoid 3'-Hydroxylase Gene and Functional Expression of the Encoded P450 Enzyme
- Gut Bacteria May Be Involved in Interactions between Plants, Herbivores and Their Predators: Microbial Biosynthesis of N-Acylglutamine Surfactants as Elicitors of Plant Volatiles
- Different Signaling Pathways Are Involved in CCKB Receptor-Mediated MAP Kinase Activation in COS-7 Cells
- Non-Specific Depurination Activity of Saporin-S6, a Ribosome-Inactivating Protein, under Acidic Conditions
- Molecular Evolution in the Hypervariable Regions of Fetuin: Comparison between Human and African Green Monkey Fetuin
