Two Differentially Regulated Class II Chitinases from Parsley
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Y. Ponath
Abstract
Two distinct cDNA clones, PcCHI1 and PcCHI2, with high sequence similarity to plant chitinases were isolated from parsley (Petroselinum crispum), expressed in Escherichia coli, and the encoded proteins functionally identified as endochitinases. Different expression patterns of the corresponding mRNAs and proteins in infected and uninfected parsley plants indicated distinct roles of the two isoforms in both pathogen defense and plant development. Infection of parsley leaf buds with Phytophthora sojae resulted in the rapid, transient and highly localized accumulation of PcCHI1 mRNA and protein around infection sites, whereas PcCHI2 mRNA and protein were systemically induced at later infection stages. Similar differences in the timing of induction were observed in elicitor-treated, suspension-cultured parsley cells. In uninfected plants, PcCHI1 mRNA was particularly abundant in the transmitting tract of healthy flowers, suggesting a role in the constitutive protection of susceptible transmitting tissue of the style against pathogen ingress and/or in the fertilization process, possibly by affecting pollen tube growth. Localization of PcCHI2 mRNA and protein in the parenchymatic collenchyme of young pedicels may indicate a function in the constitutive protection of this tissue. In addition to such distinct roles of PcCHI1 and PcCHI2 in preformed and induced pathogen defense, both chitinases may have endogenous regulatory functions in plant development.
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- To Our Authors, Readers and Subscribers
- Highlight: Plant Biological Chemistry
- The Structural Properties of Plant Peroxisomes and Their Metabolic Significance
- Active Oxygen Species as Mediators of Plant Immunity: Three Case Studies
- Post-Transcriptional Regulation of Phenylalanine Ammonia-Lyase Expression in Tobacco Following Recovery from Gene Silencing
- Two Differentially Regulated Class II Chitinases from Parsley
- IAA-Synthase, an Enzyme Complex from Arabidopsis thaliana Catalyzing the Formation of Indole-3-Acetic Acid from (S)-Tryptophan
- Topology Studies of the Chloroplast Protein Import Channel Toc75
- Plant Methionine Synthase: New Insights into Properties and Expression
- The Hepta-? -Glucoside Elicitor-Binding Proteins from Legumes Represent a Putative Receptor Family
- Octadecanoid and Jasmonate Signaling in Tomato (Lycopersicon esculentum Mill.) Leaves: Endogenous Jasmonates Do Not Induce Jasmonate Biosynthesis
- Metabolic Activity Decreases as an Adaptive Response to Low Internal Oxygen in Growing Potato Tubers
- Structure-Activity Relationships of Synthetic Analogs of Jasmonic Acid and Coronatine on Induction of Benzophenanthridine Alkaloid Accumulation in Eschscholzia californica Cell Cultures
- Identification of the Arabidopsis thaliana Flavonoid 3'-Hydroxylase Gene and Functional Expression of the Encoded P450 Enzyme
- Gut Bacteria May Be Involved in Interactions between Plants, Herbivores and Their Predators: Microbial Biosynthesis of N-Acylglutamine Surfactants as Elicitors of Plant Volatiles
- Different Signaling Pathways Are Involved in CCKB Receptor-Mediated MAP Kinase Activation in COS-7 Cells
- Non-Specific Depurination Activity of Saporin-S6, a Ribosome-Inactivating Protein, under Acidic Conditions
- Molecular Evolution in the Hypervariable Regions of Fetuin: Comparison between Human and African Green Monkey Fetuin
