Polyhistidine-Tagged Hepatitis B Core Particles as Carriers of HIV-1/gp120 Epitopes of Different HIV-1 Subtypes
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Abstract
The hepatitis B core antigen is a widely accepted carrier particle to enhance the immunogenicity of foreign epitopes. From electron cryomicroscopy, the immunodominant region between amino acid positions 79 to 81 is known to protrude from the surface of the shells. It can be replaced by heterologous sequences without interfering with the particleforming capacity in many cases. Here we have introduced various V3 sequences of the envelope protein of different subtypes (A, B, O) of HIV-1/gp120 in order to enhance their immunogenicity and broaden the immune response against the virus. To improve purification efficiency and solubility of the E. coliexpressed hybrids, six histidine residues were fused to amino acid 156. An adjustable purification scheme was utilised including denaturation, Ni[2+]NTA affinity chromatography and particle renaturation under high salt conditions, resulting in highly pure antigen preparations. The hybrids reacted specifically with sera of HIV-1- infected patients. They further induced an autologous, subtypespecific anti HIV-1 antibody response superior to that of Keyhole limpethaemocyaninecoupled peptides.
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
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- Stability of Bacteriophage T4 Short Tail Fiber
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Artikel in diesem Heft
- Interaction of E. coli Single-Stranded DNA Binding Protein (SSB) with Exonuclease I. The Carboxy-Terminus of SSB Is the Recognition Site for the Nuclease
- p38/SAPK2-Dependent Gene Expression in Jurkat T Cells
- The First Laminin G-Type Domain in the SHBG-Like Region of Protein S Contains Residues Essential for Activation of the Receptor Tyrosine Kinase Sky
- Permutation of the Active Site Motif of Tryparedoxin 2
- Structural Investigations of the Highly Flexible Recombinant Ribosomal Protein L12 from Thermotoga maritima
- Polyhistidine-Tagged Hepatitis B Core Particles as Carriers of HIV-1/gp120 Epitopes of Different HIV-1 Subtypes
- In Vitro Evolution of Ligands for HCV-Specific Serum Antibodies
- Stability of Bacteriophage T4 Short Tail Fiber
- Analysis of the RNase T1 Mediated Cleavage of an Immobilized Gapped Heteroduplex via Fluorescence Correlation Spectroscopy
- Binding of Selenoprotein P to Heparin: Characterization with Surface Plasmon Resonance
- Biotin-Avidin Microplate Assay for the Quantitative Analysis of Enzymatic Methylation of DNA by DNA Methyltransferases
