Startseite Mitochondria-Derived and Extra-Mitochondrial Human Type-1 Porin Are Identical as Revealed by Amino Acid Sequencing and Electrophysiological Characterisation
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Mitochondria-Derived and Extra-Mitochondrial Human Type-1 Porin Are Identical as Revealed by Amino Acid Sequencing and Electrophysiological Characterisation

  • Ulrike Stadtmüller , Jana Eben-Brunnen , Angela Schmid , Dörte Hesse , Simone Klebert , Hartmut D. Kratzin , Jan Hesse , Bodo Zimmermann , Susanne Reymann , Friedrich P. Thinnes , Roland Benz , Hilde Götz und Norbert Hilschmann
Veröffentlicht/Copyright: 1. Juni 2005
Biological Chemistry
Aus der Zeitschrift Band 380 Heft 12

Abstract

In mammalian cells porin channels are localised in both mitochondrial outer membranes and extra-mitochondrial membranes. We isolated mitochondria-derived porin of a human lymphoblastoid B cell line, determined its amino acid sequence and characterised its channel properties. Interestingly, the amino acid sequence of this porin preparation and, correspondingly, its electrophysiological characteristics in a reconstituted system were identical to those of ‘Porin 31HL’, the human type-1 porin purified from a crude membrane preparation of the same cell line using a different purification protocol. The results raise questions about targeting, insertion and orientation of human type-1 porin in different membranes.

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Published Online: 2005-06-01
Published in Print: 1999-12-20

Copyright (c) 1999 by Walter de Gruyter GmbH & Co. KG

Artikel in diesem Heft

  1. To Our Authors, Readers and Subscribers
  2. Editor's Note
  3. A Chimpanzee Millennium
  4. Molecular Genetics of Dopa-Responsive Dystonia
  5. In Vitro Transcription of a TATA-Less Promoter: Negative Regulation by the Not1 Protein
  6. Fast Control of DNA Replication in Response to Hypoxia and to Inhibited Protein Synthesis in CCRF-CEM and HeLa Cells
  7. The Inhibition of NF-B Activation Pathways and the Induction of Apoptosis by Dithiocarbamates in T Cells Are Blocked by the Glutathione Precursor N-Acetyl-L-Cysteine
  8. Xylose Utilisation: Cloning and Characterisation of the Xylose Reductase from Candida tenuis
  9. Xylose Utilisation: Cloning and Characterisation of the Xylitol Dehydrogenase from Galactocandida mastotermitis
  10. Thermodynamic Properties and DNA Binding of the ParD Protein from the Broad Host-Range Plasmid RK2/RP4 Killing System
  11. Dipeptidyl Peptidase III from Rat Liver Cytosol: Purification, Molecular Cloning and Immunohistochemical Localization
  12. Genomic Expansion Across the Albumin Gene Family on Human Chromosome 4q Is Directional
  13. Comparison of the Tamoxifen Regulated Chimeric Cre Recombinases MerCreMer and CreMer
  14. The Human Cathepsin F Gene a Fusion Product between an Ancestral Cathepsin and Cystatin Gene
  15. Characterization of a New Member of the TNF Family Expressed on Antigen Presenting Cells
  16. Vascular Endothelial Growth Factor (VEGF) and Its Receptors in Tumor-Bearing Dogs
  17. Molecular Cloning and Characterization of a cDNA Encoding a Transferrin Homolog from Bombyx mori
  18. Mitochondria-Derived and Extra-Mitochondrial Human Type-1 Porin Are Identical as Revealed by Amino Acid Sequencing and Electrophysiological Characterisation
  19. Acknowledgement
  20. Content Index
  21. Author Index
  22. Subject Index
https://doi.org/10.1515/BC.1999.189

Artikel in diesem Heft

  1. To Our Authors, Readers and Subscribers
  2. Editor's Note
  3. A Chimpanzee Millennium
  4. Molecular Genetics of Dopa-Responsive Dystonia
  5. In Vitro Transcription of a TATA-Less Promoter: Negative Regulation by the Not1 Protein
  6. Fast Control of DNA Replication in Response to Hypoxia and to Inhibited Protein Synthesis in CCRF-CEM and HeLa Cells
  7. The Inhibition of NF-B Activation Pathways and the Induction of Apoptosis by Dithiocarbamates in T Cells Are Blocked by the Glutathione Precursor N-Acetyl-L-Cysteine
  8. Xylose Utilisation: Cloning and Characterisation of the Xylose Reductase from Candida tenuis
  9. Xylose Utilisation: Cloning and Characterisation of the Xylitol Dehydrogenase from Galactocandida mastotermitis
  10. Thermodynamic Properties and DNA Binding of the ParD Protein from the Broad Host-Range Plasmid RK2/RP4 Killing System
  11. Dipeptidyl Peptidase III from Rat Liver Cytosol: Purification, Molecular Cloning and Immunohistochemical Localization
  12. Genomic Expansion Across the Albumin Gene Family on Human Chromosome 4q Is Directional
  13. Comparison of the Tamoxifen Regulated Chimeric Cre Recombinases MerCreMer and CreMer
  14. The Human Cathepsin F Gene a Fusion Product between an Ancestral Cathepsin and Cystatin Gene
  15. Characterization of a New Member of the TNF Family Expressed on Antigen Presenting Cells
  16. Vascular Endothelial Growth Factor (VEGF) and Its Receptors in Tumor-Bearing Dogs
  17. Molecular Cloning and Characterization of a cDNA Encoding a Transferrin Homolog from Bombyx mori
  18. Mitochondria-Derived and Extra-Mitochondrial Human Type-1 Porin Are Identical as Revealed by Amino Acid Sequencing and Electrophysiological Characterisation
  19. Acknowledgement
  20. Content Index
  21. Author Index
  22. Subject Index
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